non palindromic recognition sequences
Bill Melchior, NCTR/FDA
wmelchior at NTBTOX.NCTR.FDA.GOV
Thu Sep 24 09:19:19 EST 1992
> Does anyone know of a Restriction Endonuclease that cuts WITHIN
>a non-palindromic recognition sequence,and if so,an available source.
> New England Biolabs' Aci I (CCGC) unfortunately leaves a palin-
>dromic overhang which is unsuitable for our purposes.
AvaI CAN leave a non-palindromic overhang. It cuts once in pBR322 to leave
a 5' TCGG. The recognition sequence is CYCGRG, so it can cut at both
palindromic and non-palindromic sites. There are other enzymes listed with
similar ambiguous recognition sequences.
The opinions stated are mine, not those of NCTR or its sponsoring organizations.
Bill Melchior || OMNISCIENCE
National Center for Toxicological Research || Knowing what
Jefferson, AR 72079 || thou knowest not
(501) 543-7206 || is in a sense
WMELCHIOR at NTDOC.NCTR.FDA.GOV || from Grooks, Piet Hein
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