non palindromic recognition sequences

[Bill Melchior, NCTR/FDA]

>           Does anyone know of a Restriction Endonuclease that cuts WITHIN
>a non-palindromic recognition sequence,and if so,an available source.
>           New England Biolabs' Aci I (CCGC) unfortunately leaves a palin-
>dromic overhang which is unsuitable for our purposes.

AvaI CAN leave a non-palindromic overhang.  It cuts once in pBR322 to leave
a 5' TCGG.  The recognition sequence is CYCGRG, so it can cut at both
palindromic and non-palindromic sites.  There are other enzymes listed with
similar ambiguous recognition sequences.
________________________________________________________________________________
The opinions stated are mine, not those of NCTR or its sponsoring organizations.

Bill Melchior                                ||     OMNISCIENCE
National Center for Toxicological Research   ||    Knowing what
Jefferson, AR  72079                         ||    thou knowest not
(501) 543-7206                               ||    is in a sense
                                             ||    omniscience.
WMELCHIOR at NTDOC.NCTR.FDA.GOV                 ||       from Grooks, Piet Hein