single strand specific mutagens ( >bisulfite)

Basavaraju Shankarappa bsh at MED.PITT.EDU
Tue Sep 22 09:42:26 EST 1992

I do not whether this is what you are looking for.  
I had read about the following method for something else.
Here the ss unhybridized DNA is modified by a water soluble
compound to identify the presence of mismatch by subsequent
primer extension.  
See if this will be of any use.
Ganguly and Prockop.  Detection of single-base mutations by
reaction of DNA heteroduplexes with a water-soluble carbodiimide
followed by primer extension: application to products from the
PCR.  Nucl Acid. res. 18:3933-3939.
Good luck.
Raj Shankarappa
Univ of Pittsburgh

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