Probing native DNA on filters(??)
Paul N Hengen
pnh at fcsparc6.ncifcrf.gov
Mon Sep 21 10:30:17 EST 1992
ccy at po.CWRU.Edu (Cheung C. Yue) writes:
:...perhaps another useful technique is that of whole genome PCR
:which I think was developed by Vogelstein's lab. In that technique,
:DNA is fragmented, ligated to amplification primer-adapters, then
:subjected to binding selection on immobilized DNA binding protein.
:Bound DNA are rescued and amplified by PCR for additional rounds of
:selection. Finally selected DNA fragments are cloned and sequenced.
:Such fragments are obviously useful for screening genomic libraries.
:ccy at po.cwru.edu
Would anyone happen to have any references related to this type of
Paul N. Hengen
National Cancer Institute
Frederick Cancer Research and Development Center
Frederick, Maryland 21702-1201 USA
e-mail: pnh at ncifcrf.gov
More information about the Methods