Probing native DNA on filters(??)

Paul N Hengen pnh at fcsparc6.ncifcrf.gov
Mon Sep 21 10:30:17 EST 1992


ccy at po.CWRU.Edu (Cheung C. Yue) writes:

:...perhaps another useful technique is that of whole genome PCR
:which I think was developed by Vogelstein's lab.  In that technique,
:DNA is fragmented, ligated to amplification primer-adapters, then
:subjected to binding selection on immobilized DNA binding protein.
:Bound DNA are rescued and amplified by PCR for additional rounds of
:selection.  Finally selected DNA fragments are cloned and sequenced.
:Such fragments are obviously useful for screening genomic libraries.
:
:ccy at po.cwru.edu


Would anyone happen to have any references related to this type of
selection?

Paul N. Hengen
National Cancer Institute
Frederick Cancer Research and Development Center
Frederick, Maryland 21702-1201 USA
e-mail: pnh at ncifcrf.gov



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