Direct PCR of Lambda Libraries

Stuart Brown browns at ccu.umanitoba.ca
Fri Sep 18 11:04:36 EST 1992


In article <920914080921.4022 at UTBC01.CM.UTEXAS.EDU> READY at UTBC01.CM.UTEXAS.EDU writes:
>Dear Netters,
>
>	I recall hearing or reading of a method for PCR of a desired poly-
>nucleotide directly from a cDNA library in Lambda phage. The idea was that for
>a more-or-less known sequence, one could take a few ul of the phage, strip
>their coats and use the DNA directly in a PCR reaction. If anyone has a method
>or can point me to the reference, I would be a very happy guy (my hair is
>getting to thin for continued 32P use). If there is sufficient interest, I'll
>post a summary of responses.
>
>Thanks in advance,
>
>Mike R.
>    _______________________________________________________________________
>   | Dr. Michael P. Ready                     | READY at UTBC01.CM.UTEXAS.EDU |
>   | Clayton Foundation Biochemical Institute | Phone: (512)471-3625       |
>   | University of Texas                      | FAX:   (512)471-8696       |
>   | Austin, Texas 78737                      |                            |
>   |__________________________________________|____________________________|
>  

We do PCR from lambda clones and lambda libraries routinely.  No need to
strip off coat proteins - denaturation step of PCR must be sufficient.
-- 
Stuart M. Brown                             If you can remain cool when all 
U. of Manitoba, Dept. Plant Science         Around you are in panic,
Winnipeg, Manitoba, CANADA
browns at ccu.umanitoba.ca            Then you surely misunderstand the situation



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