IEF Western blotting ok?

Yves Bertheau bertheau at
Sun Sep 20 10:02:59 EST 1992

In article <16SEP92.21800042.0057.MUSIC at MUSICA.MCGILL.CA> CX13 at MUSICA.MCGILL.CA ("Yannick at CX13") writes:
>Howdy Netters! Does anybody if one can blot an IEF gel for subsequent
>antibody probing, the way you would do it w/a standard SDS-PAGE gel?
>My boss thinks there might be background problems. I wonder...
>Virtually yours,
>Yannick Pouliot
>Montreal Neurological Institute

After electrofocusing of 3-10 pH range of Pharmacia ampholynes we blot
using a Triethylamine-HCl pH 11 buffer.

It seems to work fine...

Hoping it is helpful for you
		Yves Bertheau
INRA INA P-G		Pathologie Vegetale	16 rue Claude Bernard	
75231 PARIS cedex 05	FRANCE	Tel +33 (1) 
Fax: +33 (1) Internet: bertheau at

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