IEF Western blotting ok?

Yves Bertheau bertheau at inapv.inapg.inra.fr
Sun Sep 20 10:02:59 EST 1992


In article <16SEP92.21800042.0057.MUSIC at MUSICA.MCGILL.CA> CX13 at MUSICA.MCGILL.CA ("Yannick at CX13") writes:
>Howdy Netters! Does anybody if one can blot an IEF gel for subsequent
>antibody probing, the way you would do it w/a standard SDS-PAGE gel?
>My boss thinks there might be background problems. I wonder...
>
>Virtually yours,
>
>Yannick Pouliot
>Montreal Neurological Institute

After electrofocusing of 3-10 pH range of Pharmacia ampholynes we blot
using a Triethylamine-HCl pH 11 buffer.

It seems to work fine...


Hoping it is helpful for you
-- 
		Yves Bertheau
INRA INA P-G		Pathologie Vegetale	16 rue Claude Bernard	
75231 PARIS cedex 05	FRANCE	Tel +33 (1) 43.31.93.97 
Fax: +33 (1) 43.31.83.82 Internet: bertheau at inapg.inra.fr



More information about the Methods mailing list