FACS of yeast
camdna at ubvmsb.cc.buffalo.edu
Fri Sep 18 13:53:00 EST 1992
In article <9209172141.AA26504 at genbank.bio.net>, RGB12955%USA.decnet at USAV01.GLAXO.COM ("USA::RGB12955") writes...
>Has anyone tried (or heard of anyone trying) to analyze and sort yeast via
>FACS. I have heard of someone doing so on yeast protoplasts, but how
>about whole cells? Are there any particular problems with it that I need to
>address before getting started?
See Srienc, Bailey, and Campbell (1985) MCB 5(7):1676-1684. They used cells
permeabilized before cytometry. I have not tried the method first hand so I
cannot comment on its ease of use, etc.
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