Probing native DNA on filters(??)
bsh at MED.PITT.EDU
Fri Sep 18 13:43:47 EST 1992
> My question may be simple, but we don't seem to have
> anyone here who has attempted this. Has anyone had any experience
> with blotting native DNA (ie. Chromosome digest, non-denatured)
> onto nylon filters and subsequently probing this DNA with a DNA
> binding protein? I understand it is simple to blot native DNA
> but will UV crosslinking fix it to the filter without having
> previously denatured it?
> One would assume that a DNA binding protein would require
> the DNA binding site to be 1) in native form and 2) free from
> binding to the filter. It would be nice if this method could
> work and be used with a chromosomal digest blot or (even better)
> a lambda bacterial library via colony hybridization.
> G. Scott Gordon
> Tufts U. - Sackler School
Is this not the same as South-Western blot;
There seems to be so many blots it seems to be confusing.
May be if somebody has a glossary of all the blot definitions
would they mind posting it.
If not, I will try to fish the meaning of different blots that I can
and may be otherS can add on as the time goes by.
Univ of Pittsburgh
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