dam- e.coli

Basavaraju Shankarappa bsh at MED.PITT.EDU
Thu Sep 17 08:48:16 EST 1992


This question is for people who have worked with dam- E.coli.
I was given a dam- strain called GM33.  When I attempted to make competent
cells by the CaCl2 procedure, the cells would aggregate kind of precipitate
the moment 100 mM CaCl2 solution is added.
Does anyone know why and how to overcome this problem.
I have attempted to use the aggregated cells, but they do  not transform
Thanks for the insight.
Raj Shankarappa
Univ of Pittsburgh.



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