Acidic polyacrylamide gels
bertheau at inapv.inapg.inra.fr
Wed Sep 16 18:32:29 EST 1992
In article <5175 at news.duke.edu> Greenleaf at bchm.biochem.duke.edu (Andrea Skantar) writes:
>I am studying protein-protein interactions as described in the post of
>Cornelius Krasel recently. In particular, I have tried the so-called
>"far-Western" blot technique, where you use labeled protein as a probe
>instead of antibody. So far I have not seen anything and I have recently
>learned that the protein I'm trying to detect has two subunits, one with
>a pI around neutral and the other at pI=10.4. So I'm not sure that this
>protein has been entering the gels I've been running in Tris-glycine at
>pH=8. Does anybody have any info on native acidic polyacrylamide gels?
>We don't have isoelectric focussing equipment. Any advice from people who
>work on histones or other basic proteins? I'd appreciate any input.
Several years ago I used for alkaline proteins a Triethylamine HCl buffer:
pH 11, it worked well in native conditions...
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