Acidic polyacrylamide gels

Yves Bertheau bertheau at inapv.inapg.inra.fr
Wed Sep 16 18:32:29 EST 1992


In article <5175 at news.duke.edu> Greenleaf at bchm.biochem.duke.edu (Andrea Skantar) writes:
>I am studying protein-protein interactions as described in the post of 
>Cornelius Krasel recently.  In particular, I have tried the so-called
>"far-Western" blot technique, where you use labeled protein as a probe
>instead of antibody.  So far I have not seen anything and I have recently
>learned that the protein I'm trying to detect has two subunits, one with
>a pI around neutral and the other at pI=10.4.  So I'm not sure that this
>protein has been entering the gels I've been running in Tris-glycine at 
>pH=8.  Does anybody have any info on native acidic polyacrylamide gels?
>We don't have isoelectric focussing equipment.  Any advice from people who
>work on histones or other basic proteins?  I'd appreciate any input.  
>
>Thanks,
>
>Andrea

Several years ago I used for alkaline proteins a Triethylamine HCl buffer: 
pH 11, it worked well in native conditions...

Good luke
-- 
		Yves Bertheau
INRA INA P-G		Pathologie Vegetale	16 rue Claude Bernard	
75231 PARIS cedex 05	FRANCE	Tel +33 (1) 43.31.93.97 
Fax: +33 (1) 43.31.83.82 Internet: bertheau at inapg.inra.fr



More information about the Methods mailing list