DAVID F. BISHOP
BISHOP at MSVAX.MSSM.EDU
Wed Sep 16 15:17:42 EST 1992
>In a previous article, SMITH_DENNIS_P at LILLY.COM ("DENNIS P. SMITH") says:
>>Need advice of how to take photograph of an oligonucleotides using UV
>>shadowing. Can anyone suggest a reliable technique? Thanks DPSMITH
>Put your gel on a piece of Saran Wrap, and lay it atop a fluorescing
>substrate (More on this below). Then use a hand illuminator to shine
>through the gel, and detect dark spots on the otherwise bright background.
>Finding the right fluorescing plate is sometimes tricky. What always works
>is the glass cellulose TLC plates that are marked "with fluorescence
>indicator"; maybe the plastic plates or the silica TLC plates work too.
The plastic-backed TLC plates that we always use work well and have a bright
green fluorescence. They are distributed by Brinkman Instruments, Inc
and are Macherey-Nagel Polygram SIL G/UV254 plates. 25/box, 20x20 cm.
The lot we are using is about 10 years old, but I presume they are still
available. They last a long time if you wrap them with plastic film.
David Bishop | EMail:Internet:Bishop at msvax.mssm.edu
Div. of Medical and Molecular Genetics | Bitnet:Bishop at msrcvax
Mount Sinai School of Medicine, Box 1203 | Phone: (212) 241-6946
Fifth Avenue and 100th Street | FAX: (212) 360-1809
New York, NY 10029 | AOL: DFBishop
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