PCR enhancers
John Alsobrook
ALSOBROOK at biomed.med.yale.edu
Tue Sep 15 12:30:48 EST 1992
In <9209101632.AA03158 at anolis.bnr.usu.edu> thsiao at ANOLIS.BNR.USU.EDU writes:
> Dear Netters,
>
> I am interested in the use of PCR enhancers. I would like to know if any one
> has experience with the use of Stratagene's Perfect Match Polymerase Enhancer.
> Is this a single-stranded DNA binding protein? What is the difference with
> USB's single-stranded DNA binding protein or GP 32? How effective are these
> products in getting better PCR yields? Any suggestion and tips will be
> appreciated.
>
> +---------------Ting H. Hsiao ---------------+
> | Dept. of Biology, Utah State University |
> | Logan, UT 84322-5303, U.S.A. |
> | PHONE: (801) 750-2549; FAX: (801) 750-1575 |
> | E-MAIL: thsiao at anolis.bnr.usu.edu |
> +--------------------------------------------+
An issue of Biotechniques (I think, not absolutely sure) a couple of years ago
revealed that Stratagene's Perfect Match is actually a 5x10E-5 M solution of
tetra-methyl-ammonium chloride.
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