Lysis of bacteria

Bj|rn Forbord bjoernf at ulrik.uio.no
Tue Sep 15 03:32:01 EST 1992


We are currently running PCR on samples consisting of a number of different
bacterial strains.  We don't know which bugs are in the samples and are
utilizing a number of primers in this endeavour.  Our main concern is this:
How do we know whether we really are able to lyse any bacterium, regardless
of cell wall composition etc.?
Can anyone out there suggest good lysing techniques that don't interfere with
PCR amplification?
--
Bjoern Forbord
(bjoernf at karius.uio.no)
Odont Inst Physiol Biochem
Dental Faculty
PO Box 1052 Blindern
N-0316 OSLO 3
Tel: +47 2 856029
Fax: +47 2 856025
Secretary: +47 2 854462



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