TYE Medium?

Michael Benedik bchs1b at Elroy.UH.EDU
Sun Sep 13 21:33:19 EST 1992


In article <13SEP199207092503 at aardvark.ucs.uoknor.edu>, broe at aardvark.ucs.uoknor.edu (Bruce Roe) writes:
>In article <1992Sep11.171500.1 at hmivax.humgen.upenn.edu>, dreyfuss at hmivax.humgen.upenn.edu writes...
>>Hello--
>> 
>>Does anyone out there know about the composition of TYE medium for growing
>>E.coli TG1 cells?
>> 
>>Thanks,
>>Jeong-Kook Kim
>>Howard Hughes Medical Institute/University of Pennsylvania
>>Internet:  dreyfuss at hmivax.humgen.upenn.edu
>>(215)898-0172
>
>Hi,
>	The following is what we use for TYE plates and TYE medium:
>
>TYE plates:  
>       10 g. of Bacto-tryptone, 5 g. of yeast extract, 8 g. of NaCl,
>       15 g. of nutrient agar, distilled water to 1 liter; autoclave
>       to sterilize.
>       Cool to 55 deg C, add antibiotic if desired, pour into sterile
>       petri dishes.  (approx. 20 ml/plate)
>
>TYE medium:
>       Same as TYE plates but without any agar.
>
>  - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
> \  Bruce A. Roe                     Dept. Chemistry and Biochemistry /
> /  BROE at aardvark.ucs.uoknor.edu     University of Oklahoma           \
>  - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Bruce,
	I think there is a typo in your response. Are you sure you
mean to say 15g nutrient agar. Don't you just mean plain agar??
Nutrient agar is already a rich media all by itself. 

	---------------------------------------------------
	 Michael Benedik
	 Department of Biochemical and Biophysical Sciences
	 University of Houston
	 INTERNET: Benedik at UH.EDU	BITNET: Benedik at UHOU



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