UV Shadowing

Bruce Roe broe at aardvark.ucs.uoknor.edu
Sun Sep 13 06:58:00 EST 1992


In article <18qm5pINNscj at usenet.INS.CWRU.Edu>, djt2 at po.CWRU.Edu (Dennis J. Templeton) writes...
> 
>In a previous article, SMITH_DENNIS_P at LILLY.COM ("DENNIS P. SMITH") says:
> 
>>Need advice of how to take photograph of an oligonucleotides using UV
>>shadowing. Can anyone suggest a reliable technique? Thanks DPSMITH
> 
>Put your gel on a piece of Saran Wrap, and lay it atop a fluorescing
>substrate (More on this below).  Then use a hand illuminator to shine
>through the gel, and detect dark spots on the otherwise bright background.
> 
>Finding the right fluorescing plate is sometimes tricky.  What always works
>is the glass cellulose TLC plates that are marked "with fluorescence
>indicator"; maybe the plastic plates or the silica TLC plates work too.  We
>use instead an intensifying screen, but it turns out that many screens
>don't work (they don't fluoresce brightly)... we tried about 15 and only
>one (an old one with lots of cracks) works for us.  
> 
>If you use the glass fluorescing plates, you can put the gel on a
>transilluminator, cover with Saran, then place the plate over and view from
>the top, i.e. the back of the plate.
> 
>good luck,  dennis

We use a similar procedure with plastic-backed silica TLC plates "with
fluorescence indicator and they work fine.
-- bruce
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 \  Bruce A. Roe                     Dept. Chemistry and Biochemistry /
 /  BROE at aardvark.ucs.uoknor.edu     University of Oklahoma           \
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