expression vectors for proteins

[Rod Bonfiglioli]

does anyone have any experience of using expression vectors for largish
proteins, i am trying tofind one that will take inserts of up to 3 Kb to
express proteins of the 100 Kd range.  i have tried pGEX vectors, and they
dont seem to want do, anyone got any suggestions please?

thanx in advance

rod bonfiglioli
email rbonfigl at schooner.waite.adelaide.edu.au