DNA methylation

Martin Kennedy cytogen at chmeds.ac.nz
Wed Sep 9 17:08:08 EST 1992


In article <1992Sep9.150805.11081 at gserv1.dl.ac.uk>, sbhattac at crc.ac.uk (Dr. Shoumo Bhattacharya B1 061) writes:
> Hi 
> 
> Does anyone know of methods to demethylate methylated genes
> in tissue culture cells, so that they are accessible to 
> trans-acting factors?  I thought of growing them in 
> azacytidine, has anyone got a successful protocol for this?
> 
> Many thanks.
> 
> Shoumo
> 
> Dr. Shoumo Bhattacharya				
> MRC Molecular Medicine Group
> Royal Postgraduate Medical School
> Hammersmith Hospital, DuCane Road London W12 0NN


Shoumo-

The following reference might be helpful:

Dobkin et al (1987)  PFGE of human DNA: 5-azacytidine improves restricition
Nucl. Acids Res. 15, 3183.

Cheers,

Martin



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