DNA methylation

[Dr. Shoumo Bhattacharya B1 061]

Hi 

Does anyone know of methods to demethylate methylated genes
in tissue culture cells, so that they are accessible to 
trans-acting factors?  I thought of growing them in 
azacytidine, has anyone got a successful protocol for this?

Many thanks.

Shoumo

Dr. Shoumo Bhattacharya				
MRC Molecular Medicine Group
Royal Postgraduate Medical School
Hammersmith Hospital, DuCane Road London W12 0NN