Electroblotting

Larry Hale lhale at upei.ca
Tue Sep 1 09:06:24 EST 1992


Can anyone suggest a place to find a reliable protocol for transfer of
double stranded DNA from SDS-PAGE gels to an appropriate medium (hopefully
nylon membranes)? I have a new unit from EC Apparatus (EC-217), but the 
manuals are not terribly helpful. In particular, I need to know the steps 
required (if any) required to prepare the DNA for probe hybridization from 
such a gel.

Thanks, L.Hale, UPEI, Charlottetown, Canada



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