Photographing Coomassie-stained Gels
Bill Melchior, NCTR/FDA
wmelchior at NTBTOX.NCTR.FDA.GOV
Tue Sep 29 11:26:41 EST 1992
>does anyone have the appropriate photographic settings/conditions to get
>good quality black&white 35mm shots of Coomassie Blue-stained PAGE gels?
> - filters for contrast?
> - regular B&W film (100/125 ASA) vs. high contrast?
> - backlighting (lightbox) preferred?
> - instructions for photoprocessing prints
>we are familiar with the Kodak self-processing system but want negatives of
>the gel as well.
MANY years ago I took photos of Coomassie gels, using Polaroid's P/N 55 film
(medium contrast) to get both prints and negatives; we already had an MP-4
camera which took the film. The only problem was the relatively slow speed
(ISO 50). If you actually want slides, Polaroid has slide holders. We used
an red-orange (Wratten 23A) filter for contrast.
The opinions stated are mine, not those of NCTR or its sponsoring organizations.
Bill Melchior || OMNISCIENCE
National Center for Toxicological Research || Knowing what
Jefferson, AR 72079 || thou knowest not
(501) 543-7206 || is in a sense
WMELCHIOR at NTDOC.NCTR.FDA.GOV || from Grooks, Piet Hein
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