Photographing Coomassie-stained Gels
ED at micro.uct.ac.za
Wed Sep 30 05:06:50 EST 1992
> Dear Netters,
> does anyone have the appropriate photographic settings/conditions to get
> good quality black&white 35mm shots of Coomassie Blue-stained PAGE gels?
> - filters for contrast?
> - regular B&W film (100/125 ASA) vs. high contrast?
> - backlighting (lightbox) preferred?
> - instructions for photoprocessing prints (in regard to bracketed
> exposure settings to see effect of lighter/darker exposure
> since autoexposure print processing would make all of your
> bracketed shots look the same)
1) Kodak 1A red filter
2) ordinary B&W film, 100/125 ASA
3) on a diffuser light box (ie. backlit thru a diffuser screen)
4) deliberately bracket exposures manually, or take 3 shots on
autoexposure but use the compensation dial (if you have one) to over- and
5) print on no. 3-5 to accentuate contrast, use hardest-looking neg with
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