Methods September 1992 Archives by author

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Starting: Tue Sep 1 08:21:33 EST 1992
Ending: Wed Sep 30 18:30:22 EST 1992
Messages: 351

Designing oligos for cDNA cloning Dr. Shoumo Bhattacharya B1 061
Epstein Barr Dr. Shoumo Bhattacharya B1 061
DNA methylation Dr. Shoumo Bhattacharya B1 061
DNA methylation Dr. Shoumo Bhattacharya B1 061
Looking for a good oligo Dr. Shoumo Bhattacharya B1 061
PCR with E. Coli ANSIDHP at EARN.OSUCC
Chromosomes on disk(ette)? David A. Adler
Size of exons and introns? Dr. N.A. Affara
Partial digestions with ApaL1 - help!! Ashok Aiyar
Partial digestions with ApaL1 - help!! - THANK YOU Ashok Aiyar
Epstein Barr Ashok Aiyar
dam- e.coli Ashok Aiyar
Epstein Barr John Alsobrook
PCR enhancers John Alsobrook
PCR primers containing Inosine? Dr. S.A.J.R. Aparicio
PCR buffers Dr. S.A.J.R. Aparicio
FinI enzyme info? BACKD at QUCDN.QueensU.CA
Luciferase activity by scintillation counting? BACKD at QUCDN.QueensU.CA
ammonia cleavage from proteins BECKERM at SIMSC.BITNET
AMMONIA CLEAVAGE FROM PROTEINS BECKERM at SIMSC.EARN
Cracked PAGE gels BI1RGW at ibm.shef.ac.uk
UV Shadowing DAVID F. BISHOP
MICROPIPETE CALIBRATION DAVID F. BISHOP
HIV BMA14432 at VAX1.UTULSA.EDU
lambda won't cut Donald W. Back
PBR322 - maximizing yields Michael Benedik
non-specific cleavage of X-gal Michael Benedik
Subtracted mRNA probes for bacteria? Michael Benedik
TYE Medium? Michael Benedik
Lambda EMBL Michael Benedik
Re Bcl1 Michael Benedik
cheap growth media other than Difco Michael Benedik
MICROPIPETE CALIBRATION ? Michael Benedik
Immunoprecipitation (again!) Andrew William Berry
Designing oligos for cDNA cloning Yves Bertheau
Acidic polyacrylamide gels Yves Bertheau
IEF Western blotting ok? Yves Bertheau
AT Biochem - address etc?? Dr. R.J. Beynon
Enzyme Reaction Rate Dr. R.J. Beynon
fin i enzyme info? Bill Melchior, NCTR/FDA
Modified Amino Acids - help Bill Melchior, NCTR/FDA
Quantitative Autoradiography??? Bill Melchior, NCTR/FDA
ASAP Bill Melchior, NCTR/FDA
Epstein Barr Bill Melchior, NCTR/FDA
Cryofreezers (-20C) Bill Melchior, NCTR/FDA
MICROPIPETE CALIBRATION ? Bill Melchior, NCTR/FDA
substitute PCR Gems Bill Melchior, NCTR/FDA
non palindromic recognition sequences Bill Melchior, NCTR/FDA
Photographing Coomassie-stained Gels Bill Melchior, NCTR/FDA
MICROPIPETE CALIBRATION ? Al Binnie
Sequencing PCR Products - Best way? Dr. Peter T. Boag
Xho I: A Bad Actor? Thon de Boer
expression vectors for proteins Rod Bonfiglioli
graft pcr, help needed******** Rod Bonfiglioli
correction to PCR message Rod Bonfiglioli
BclI Toby Bradshaw
Televised Symposium: Luciferase as a Real-Time Reporter of Gene Expression Thomas M. Breeden
PCR buffers Stuart Brown
cDNA library kits Stuart Brown
direct phage sequencing Stuart Brown
Direct PCR of Lambda Libraries Stuart Brown
Tritiated Chloramphenicol Source Brian Brunk
PCR with E. Coli Bruce Byrne
HIV Bruce Byrne
phage sequencing CALCIUM at FRCICT81.EARN
direct phage sequencing CALCIUM at FRCICT81.EARN
URGENT: re-use of hyb solutions CAMPBELL at EARN.FRCICT81
non palindromic recognition sequences CLBGRM at PATERSON.CHRISTIE-HOSPITAL.MANCHESTER.AC.UK
Leukocyte Marker absent in platelets George W Chacko
Leukocyte Marker absent in platelets George W Chacko
cheap growth media other than Difco Sylvain Chamberland
BclI Mark D. Van Cleve
BclI Mark D. Van Cleve
Epstein Barr Robert Coelen
Virology textbook Robert Coelen
2809 re: Epstein Barr (Anthony Davies) Robert Coelen
MICROPIPETE CALIBRATION ? Tod Critchlow
Formaldehyde and DNA Tod Critchlow
custom made cDNA libraries DMEIER at mis.mcw.edu
Help: Transfection into mammalian cells Sean Davidson
LUCIFERASE EXPRESSION Sean Davidson
Epstein Barr Anthony Davies
PCR enhancers Dan Diaz
bionet-news EXMED1::KARABINOS
ssDNA sequencing preps: NaOAc or NOT ? the End
Getting serious about Lambda DNA. the End
MICROPIPETE CALIBRATION ? the End
p56lck FGARBRECHT at mis.mcw.edu
cracked PAGE gels FGARBRECHT at mis.mcw.edu
MICROPIPETE CALIBRATION ? WAGNER FONTES
T cell epitopes FRANK,BART
lambda won't cut Martin Farrall
MICROPIPETTE CALIBRATION Wagner Fontes
Lysis of bacteria Bj|rn Forbord
Probing native DNA on filters(??) GIDEON S. GORDON
MICROPIPETE CALIBRATION ? Anita Gould
ssDNA sequencing preps: NaOAc or NOT ? Jim Graham
single strand specific mutagens ( >bisulfite) Jim Graham
MICROPIPETE CALIBRATION ? Mark Guiltinan
Electroblotting Larry Hale
Mol. Wght. prediction software John Hardham
Ligation trouble... John Hardham
ASAP John Hardham
Partial digestions with ApaL1 - help!! Paul N Hengen
screening phagemid libraries Paul N Hengen
Probing native DNA on filters(??) Paul N Hengen
MICROPIPETE CALIBRATION ? Paul N Hengen
MICROPIPETTE CALIBRATION (FAQ contributions) Paul N Hengen
UV Shadowing Paul N Hengen
cm amplification? (FAQ!!) Paul N Hengen
Enzyme Reaction Rate Jun Honda
screening phagemid libraries Robert Horton
Acidic polyacrylamide gels Robert Horton
mRNA from bovine PBMC Robert Horton
AMMONIA CLEAVAGE FROM PROTEINS Robert Horton
graft pcr, help needed******** Robert Horton
Acidic polyacrylamide gels Robert Horton
PCR enhancers Robert Horton
PCR enhancers Ting H. Hsiao
Help: Transfection into mammalian cells Jing Huang
Lambda won't cut Jing Huang
Primer compilation Richard J. Hughes
Chembiomed Synsorb J.W.
Oligosaccharides Jen-Wei
N-ter labelling of proteins Craig Justman
mRNA from bovine PBMC KENNEDY, DEBBIE A
FACS of yeast Stephen Kelley
FACS of yeast Stephen Kelley
ssDNA sequencing preps: NaOAc or NOT ?READ/NEXT Martin Kennedy
DNA methylation Martin Kennedy
lambda won't cut Martin Kennedy
ASAP Martin Kennedy
lambda digest: the pen-ultimate solution ? Martin Kennedy
ssDNA sequencing preps: NaOAc or NOT ? Klaus.Matthaei at anu.edu.au
Cryofreezers (-20C) - any problems with yours? Klaus.Matthaei at anu.edu.au
MICROPIPETTE CLEANING Klaus.Matthaei at anu.edu.au
Sequence of Bluescript Klaus.Matthaei at anu.edu.au
Genebank on CDROM? Klaus.Matthaei at anu.edu.au
hybridization problems Klaus.Matthaei at anu.edu.au
Luciferase activity by scintillation counting? Stephen Klautky
No subject Mike Klopfer
reconstitutions of proteins in a bilipid membrane Mike Klopfer
33p Steven J. Knapp
p33 Steven J. Knapp
RE-use of Hyb solutions Dave Knecht
Hybridization Incubators Dave Knecht
dam(n)- e.coli Klaus v. Knoblauch
Protein-protein interactions: summary Cornelius Krasel
long-ranger gels? Joel A. Kreps
Chromosomes on disk(ette)? Ari-Pekka Kvist
help secretion vectors LOWDEN,Darren
Oligosaccharides Joseph T.Y. Lau
Chromosomes on disk(ette)? Heikki Lehvaslaiho
Chromosomes on disk(ette)? Heikki Lehvaslaiho
VisiGel Bob Ludwig
re* re-use of hyb solutions MBJMR at UK.AC.DARESBURY.DLVH
commercial cDNA library MBWEN at CCVAX.AS.EDU.TW
Northern with Digoxigenin MIKE::PECHAN
pam tables MURPHY at JII.AFRC.AC.UK
cheap growth media other than Difco Morris F. Manolson
ftp FAQ files S. Marshall
PCR primers containing Inosine? Ontiretse Madisa Mine
Re Bcl1, and dam- strains Peter M. Muriana
Cryofreezers (-20C) - any problems with yours? Peter M. Muriana
Photographing Coomassie-stained Gels Peter M. Muriana
Summary: -20C Cryofreezer vs. Home freezers? Peter M. Muriana
Xho I: A Bad Actor? Ola Myklebost
Partial digestions with ApaL1 - help!! Darren Natale
BclI Darren Natale
BclI Darren Natale
FACS of yeast Darren Natale
Re Bcl1, and dam- strains Pamela Norton
RT-PCR w/ cell lysates? Darin O'Brien
info needed on mutagenesis kit Francis Ouellette
Long Ranger (tm) gels Number Ten Ox
Re Bcl1 PHILLIP_SANMIGUEL
gene amplification Dr Huaizheng Peng
cDNA library kits Andy Phillips
Dratagene Affinity Screening Kit Andy Phillips
UV shadowing Andy Phillips
Looking for a good oligo design program. Help! Marlon Pragnell
Direct PCR of Lambda Libraries READY at UTBC01.CM.UTEXAS.EDU
Re Bcl1 READY at UTBC01.CM.UTEXAS.EDU
Quantizing immobilization - any shortcuts? REE700A at MAINE.MAINE.EDU
TAQ as reverse transcritase RKOGEL at UCRVM2.BITNET
SDS PAGE, additives and elutions Stuart E Ravnik
Umea Drosophila stocks Peter Rice
hydrolysis of hydrophobic proteins David A Rintoul
N-ter labelling of proteins Keith Robison
UV Shadowing Bruce Roe
TYE Medium? Bruce Roe
Macintosh Sequence Aligner/Editor? Bruce Roe
alignment, etc. software Bruce Roe
Quantitative Autoradiography??? Robert Roxby
Macintosh Sequence Aligner/Editor? Robert Rumpf
GeneWorks Robert Rumpf
Genebank on CDROM? Robert Rumpf
Probing native DNA on filters(??) Ed Rybicki
Stratagene Quick-Hyb Ed Rybicki
Photographing Coomassie-stained Gels Ed Rybicki
UV Shadowing DENNIS P. SMITH
Table Top Centrifuge DENNIS P. SMITH
PCR buffers Loryn Sellner
Tacking down cells Basavaraju Shankarappa
Subtracted mRNA probes for bacteria? Basavaraju Shankarappa
dye-term removal after cycle seq rxns Basavaraju Shankarappa
ASAP Basavaraju Shankarappa
AT Biochem - address etc?? Basavaraju Shankarappa
Frozen competent RecA- strains Basavaraju Shankarappa
graft pcr, help needed******** Basavaraju Shankarappa
dam- e.coli Basavaraju Shankarappa
Probing native DNA on filters(??) Basavaraju Shankarappa
chromosome 21 Basavaraju Shankarappa
single strand specific mutagens ( >bisulfite) Basavaraju Shankarappa
HOT-START Basavaraju Shankarappa
Looking for a good oligo design program. Help! Basavaraju Shankarappa
numbers of mRNA transcripts Jiang Shunlin
Acidic polyacrylamide gels Andrea Skantar
expression vectors for proteins Andrea Skantar
Xho I: A Bad Actor? A.K. Sweatman
BclI and BamHI ligation Jarmo Niemi TUY
Partial digestions with ApaL1 - help!! Tao Tao
beta-lactamase assay, how and what substrate to use? Tao Tao
cm amplification? Tao Tao
re N's with DIG Euan R. Taylor
Mac Word and Excel templates for NIH grant forms-(about) Dennis J. Templeton
Templates for NIH grant forms are in this file Dennis J. Templeton
screening phagemid libraries Dennis J. Templeton
UV Shadowing Dennis J. Templeton
BclI Dennis J. Templeton
cm amplification Dennis J. Templeton
BclI Dennis J. Templeton
UV Shadowing Dennis J. Templeton
SDS PAGE gels that never crack. Trust me. Dennis J. Templeton
BclI and BamHI ligation Dennis J. Templeton
Genebank on CDROM? Dennis J. Templeton
Sequence of Bluescript Martti Tolvanen
PCR with E. Coli Gabriel Trueba
Lambda EMBL Gabriel Trueba
looking for Mr. Bert Gold, Bob Distel, Ken Kleen URA1335 at FRCITI51.BITNET
BclI and BamHI ligation USA::AWS4270
N2 storage of murine cell lines USA::LLR8156
cDNA library kits USA::LLR8156
FACS of yeast USA::RGB12955
(none) Prince of Wales Endocrinology Unit
LUCIFERASE EXPRESSION Prince of Wales Endocrinology Unit
X-GAL CLEAVAGE BY V. CHOLERAE Dr. Warren Wakarchuk
hydrolysis of hydrophobic proteins Dr. Warren Wakarchuk
GF/C filters for ssDNA Dr. Warren Wakarchuk
non-specific cleavage of X-gal Warren Wakarchuk
substitute PCR Gems Nigel Walker
PCR1 and pSI 4001 Daryl Webb
MODIFIED AMINO ACIDS - HELP!!! Wolfgang Wegloehner
CORRECTION MODIFIED AMINO ACIDS Wolfgang Wegloehner
dye-term removal after cycle seq rxns Jeremy Weinman
pMX Vector Nick White
alignment, etc. software Rick Wilson
TA Cloning kits Elli Wurtzel
IEF Western blotting ok? Yannick at CX13
Probing native DNA on filters(??) Cheung C. Yue
Blot name conventions (was Probing native DNA on filters(??) Cheung C. Yue
microsatellites van der Zeijst
PCR of ATCC samples Christian Zwieb
transcription magic Christian Zwieb
AT Biochem - address etc?? Klaus Schumann [Biologie]
DNA labelling kits afc at gnv.ifas.ufl.edu
Sequence gel problems afc at gnv.ifas.ufl.edu
MICROPIPETTE CLEANING afc at gnv.ifas.ufl.edu
dna electrophoresis alex at RUHIG.LLNL.GOV
re ligation trouble batc at ac.dal.ca
Cycle Sequencing Problems batc at ac.dal.ca
Cycle sequencing questions batc at ac.dal.ca
Help is needed batc at ac.dal.ca
Messages are received. Thanks a lot batc at ac.dal.ca
References on Amino Acids, bcrosby at pbi.lan.nrc.ca
DNA labelling kits bhjelle at carina.unm.edu
Xho I: A Bad Actor? bhjelle at carina.unm.edu
Leukocyte Marker absent in platelets bhjelle at carina.unm.edu
Chemiluminescent sequencing bionet
Dry Gel Hybridizations bishop at MAP.MARC.USDA.GOV
Help secretion vectors botdjl at lure.latrobe.edu.au
Xho I: A Bad Actor? braam at DINGO.RICE.EDU
UV Shadowing bugg at mbcf.stjude.org
Difficulties: M13 cloning chai_z at wehi.edu.au
lambda won't cut chai_z at wehi.edu.au
M13 Cloning chai_z at wehi.edu.au
lambda won't cut chris
fin i enzyme info? michael coyne
cm amplification michael coyne
cm amplification? michael coyne
X-GAL CLEAVAGE BY V. CHOLERAE coyne at uctvax.uct.ac.za
non-specific cleavage of X-gal coyne at uctvax.uct.ac.za
Epstein Barr Johan deBoer
mRNA from fish liver Johan deBoer
Promotor Database Johan deBoer
TYE Medium? dreyfuss at hmivax.humgen.upenn.edu
Frozen competent RecA- strains frist at ccu.umanitoba.ca
Cellophane frist at ccu.umanitoba.ca
Tacking down cells fulton at binah.cc.brandeis.edu
PVDF as a Southern Support fzhedric at hamlet.ucdavis.edu
UV Shadowing ggloor at kean.ucs.mun.ca
Genomic DNA purification system inoue at ACS.UCALGARY.CA
Cellophane jhu at RESUNIX.RI.SICKKIDS.ON.CA
33-P: how well does it work? jmv at canctr.mc.duke.edu
Cracked PAGE gels kaya at mscf.med.upenn.edu
Cracked PAGE gels kaya at mscf.med.upenn.edu
Photographing Coomasie-stained Gels kaya at mscf.med.upenn.edu
Salt ppt of RNA kim at m44.unm.edu
hot starts kim at m44.unm.edu
Stratagene Quick-Hyb kim at m44.unm.edu
Sequence gel problems lev at DFCIAIDS.DFCI.HARVARD.EDU
MICROPIPETE CALIBRATION ? lev at DFCIAIDS.DFCI.HARVARD.EDU
Xho ligation problems mangalam at SALK-SC2.SDSC.EDU
Blot Name Conventions (was Probing native DNA on filters) mangalam at SALK-SC2.SDSC.EDU
Primer compilation mangalam at SALK-SC2.SDSC.EDU
Clonetech's Bluemid mball at ac.dal.ca
lambda won't cut mcgraw%gandal.dnet at SERVER.UGA.EDU
chloramphenicol amplification mic225p at vaxc.cc.monash.edu.au
Frozen competent RecA- strains mic236u at vaxc.cc.monash.edu.au
lovastatin yukio nagano
Ligation of Bcl1 & BamH1 navot1 at agri.huji.ac.il
Acidic polyacrylamide gels power at mbcf.stjude.org
Looking for a good oligo rpgrant at vax.path.ox.ac.uk
hot starts scolnipa at esvax.dnet.dupont.com
dye-term removal after cycle seq rxns donald seto
chromosome 21 donald seto
NCTC 1469 cell-line skrshaw at susssys1.reading.ac.uk
HgiE II skspoidn at susssys1.reading.ac.uk
HgiE II skspoidn at susssys1.reading.ac.uk
re ligation trouble skspoidn at susssys1.reading.ac.uk
ASAP skspoidn at susssys1.reading.ac.uk
eppstein barr virus / herpes viruses suter at VAX.MPIZ-KOELN.MPG.dbp.de
sph1 suter at VAX.MPIZ-KOELN.MPG.dbp.de
contaminant suter at VAX.MPIZ-KOELN.MPG.dbp.de
lambda digest: the pen-ultimate solution ? suter at VAX.MPIZ-KOELN.MPG.dbp.de
some more contaminants suter at VAX.MPIZ-KOELN.MPG.dbp.de
colony hybridization suter at VAX.MPIZ-KOELN.MPG.dbp.de
pcr gems, why use them ? suter at VAX.MPIZ-KOELN.MPG.dbp.de
cm amplification oof puc related plasmids suter at VAX.MPIZ-KOELN.MPG.dbp.de
freezers twomey at helix.nih.gov
hybridization problems vewart at kean.ucs.mun.ca
Ligation trouble... wetsel_r at wums.wustl.edu
Sequence gel problems wetsel_r at wums.wustl.edu
MICROPIPETE CALIBRATION ? wetsel_r at wums.wustl.edu
PCR enhancers wetsel_r at wums.wustl.edu
Xho I: A Bad Actor? ww40
MICROPIPETE CALIBRATION ? ww40
Long Ranger (tm) gels ww40
Hgie II Dan Jacobson x-8453
T cell epitopes Dan Jacobson x-8453
PCR Primer Design programs (longish) Dan Jacobson x-8453

Last message date: Wed Sep 30 18:30:22 EST 1992
Archived on: Sun Mar 6 19:45:36 EST 2005

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