Methods September 1992 Archives by thread

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Starting: Tue Sep 1 08:21:33 EST 1992
Ending: Wed Sep 30 18:30:22 EST 1992
Messages: 351

• X-GAL CLEAVAGE BY V. CHOLERAE   coyne at uctvax.uct.ac.za
• Electroblotting   Larry Hale
• Partial digestions with ApaL1 - help!!   Ashok Aiyar
• Partial digestions with ApaL1 - help!!   Paul N Hengen
• Partial digestions with ApaL1 - help!!   Darren Natale
• Partial digestions with ApaL1 - help!!   Tao Tao
• X-GAL CLEAVAGE BY V. CHOLERAE   Dr. Warren Wakarchuk
• freezers   twomey at helix.nih.gov
• fin i enzyme info?   michael coyne
• fin i enzyme info?   Bill Melchior, NCTR/FDA
• Designing oligos for cDNA cloning   Dr. Shoumo Bhattacharya B1 061
• FinI enzyme info?   BACKD at QUCDN.QueensU.CA
• Partial digestions with ApaL1 - help!! - THANK YOU   Ashok Aiyar
• URGENT: re-use of hyb solutions   CAMPBELL at EARN.FRCICT81
• N2 storage of murine cell lines   USA::LLR8156
• Oligosaccharides   Jen-Wei
• PCR primers containing Inosine?   Ontiretse Madisa Mine
• PCR buffers   Loryn Sellner
• Protein-protein interactions: summary   Cornelius Krasel
• PCR primers containing Inosine?   Dr. S.A.J.R. Aparicio
• PCR buffers   Dr. S.A.J.R. Aparicio
• Oligosaccharides   Joseph T.Y. Lau
• hydrolysis of hydrophobic proteins   David A Rintoul
• re* re-use of hyb solutions   MBJMR at UK.AC.DARESBURY.DLVH
• PBR322 - maximizing yields   Michael Benedik
• Mol. Wght. prediction software   John Hardham
• cDNA library kits   Andy Phillips
• References on Amino Acids,   bcrosby at pbi.lan.nrc.ca
• Luciferase activity by scintillation counting?   Stephen Klautky
• VisiGel   Bob Ludwig
• Chembiomed Synsorb   J.W.
• hydrolysis of hydrophobic proteins   Dr. Warren Wakarchuk
• Mac Word and Excel templates for NIH grant forms-(about)   Dennis J. Templeton
• Templates for NIH grant forms are in this file   Dennis J. Templeton
• PCR with E. Coli   ANSIDHP at EARN.OSUCC
• Luciferase activity by scintillation counting?   BACKD at QUCDN.QueensU.CA
• MODIFIED AMINO ACIDS - HELP!!!   Wolfgang Wegloehner
• PCR buffers   Stuart Brown
• cDNA library kits   Stuart Brown
• CORRECTION MODIFIED AMINO ACIDS   Wolfgang Wegloehner
• DNA labelling kits   afc at gnv.ifas.ufl.edu
• Quantizing immobilization - any shortcuts?   REE700A at MAINE.MAINE.EDU
• PCR with E. Coli   Bruce Byrne
• Modified Amino Acids - help   Bill Melchior, NCTR/FDA
• DNA labelling kits   bhjelle at carina.unm.edu
• Tacking down cells   fulton at binah.cc.brandeis.edu
• cDNA library kits   USA::LLR8156
• Epstein Barr   Johan deBoer
• ammonia cleavage from proteins   BECKERM at SIMSC.BITNET
• PCR with E. Coli   Gabriel Trueba
• RE-use of Hyb solutions   Dave Knecht
• Frozen competent RecA- strains   mic236u at vaxc.cc.monash.edu.au
• lovastatin   yukio nagano
• Difficulties: M13 cloning   chai_z at wehi.edu.au
• pMX Vector   Nick White
• HgiE II   skspoidn at susssys1.reading.ac.uk
• Hgie II   Dan Jacobson x-8453
• No subject   Mike Klopfer
• Epstein Barr   Robert Coelen
• ftp FAQ files   S. Marshall
• HgiE II   skspoidn at susssys1.reading.ac.uk
• pam tables   MURPHY at JII.AFRC.AC.UK
• Ligation trouble...   wetsel_r at wums.wustl.edu
• eppstein barr virus / herpes viruses   suter at VAX.MPIZ-KOELN.MPG.dbp.de
• Acidic polyacrylamide gels   Andrea Skantar
• AT Biochem - address etc??   Dr. R.J. Beynon
• reconstitutions of proteins in a bilipid membrane   Mike Klopfer
• Epstein Barr   Dr. Shoumo Bhattacharya B1 061
• custom made cDNA libraries   DMEIER at mis.mcw.edu
• Tacking down cells   Basavaraju Shankarappa
• ssDNA sequencing preps: NaOAc or NOT ?   Jim Graham
• Help: Transfection into mammalian cells   Jing Huang
• ssDNA sequencing preps: NaOAc or NOT ?   Klaus.Matthaei at anu.edu.au
• Virology textbook   Robert Coelen
• Epstein Barr   Anthony Davies
• re ligation trouble   skspoidn at susssys1.reading.ac.uk
• non-specific cleavage of X-gal   coyne at uctvax.uct.ac.za
• Ligation trouble...   John Hardham
• Chemiluminescent sequencing   bionet
• sph1   suter at VAX.MPIZ-KOELN.MPG.dbp.de
• contaminant   suter at VAX.MPIZ-KOELN.MPG.dbp.de
• lambda won't cut   mcgraw%gandal.dnet at SERVER.UGA.EDU
• DNA methylation   Dr. Shoumo Bhattacharya B1 061
• Quantitative Autoradiography???   Robert Roxby
• ASAP   skspoidn at susssys1.reading.ac.uk
• Quantitative Autoradiography???   Bill Melchior, NCTR/FDA
• ASAP   Bill Melchior, NCTR/FDA
• ASAP   John Hardham
• ssDNA sequencing preps: NaOAc or NOT ?   the End
• lambda won't cut   Donald W. Back
• re ligation trouble   batc at ac.dal.ca
• ssDNA sequencing preps: NaOAc or NOT ?READ/NEXT   Martin Kennedy
• DNA methylation   Martin Kennedy
• lambda won't cut   Martin Kennedy
• dye-term removal after cycle seq rxns   Jeremy Weinman
• non-specific cleavage of X-gal   Michael Benedik
• Subtracted mRNA probes for bacteria?   Michael Benedik
• lambda won't cut   Martin Farrall
• Subtracted mRNA probes for bacteria?   Basavaraju Shankarappa
• dye-term removal after cycle seq rxns   Basavaraju Shankarappa
• ASAP   Basavaraju Shankarappa
• AT Biochem - address etc??   Basavaraju Shankarappa
• PCR enhancers   Ting H. Hsiao
• lambda digest: the pen-ultimate solution ?   suter at VAX.MPIZ-KOELN.MPG.dbp.de
• Frozen competent RecA- strains   frist at ccu.umanitoba.ca
• screening phagemid libraries   Robert Horton
• some more contaminants   suter at VAX.MPIZ-KOELN.MPG.dbp.de
• Lambda won't cut   Jing Huang
• Frozen competent RecA- strains   Basavaraju Shankarappa
• UV Shadowing   DENNIS P. SMITH
• Salt ppt of RNA   kim at m44.unm.edu
• lambda won't cut   chris
• 33-P: how well does it work?   jmv at canctr.mc.duke.edu
• dna electrophoresis   alex at RUHIG.LLNL.GOV
• non-specific cleavage of X-gal   Warren Wakarchuk
• ASAP   Martin Kennedy
• 2809 re: Epstein Barr (Anthony Davies)   Robert Coelen
• AT Biochem - address etc??   Klaus Schumann [Biologie]
• dye-term removal after cycle seq rxns   donald seto
• screening phagemid libraries   Paul N Hengen
• phage sequencing   CALCIUM at FRCICT81.EARN
• GF/C filters for ssDNA   Dr. Warren Wakarchuk
• Acidic polyacrylamide gels   power at mbcf.stjude.org
• screening phagemid libraries   Dennis J. Templeton
• UV Shadowing   Dennis J. Templeton
• mRNA from bovine PBMC   KENNEDY, DEBBIE A
• TYE Medium?   dreyfuss at hmivax.humgen.upenn.edu
• lambda digest: the pen-ultimate solution ?   Martin Kennedy
• NCTC 1469 cell-line   skrshaw at susssys1.reading.ac.uk
• UV Shadowing   Bruce Roe
• TYE Medium?   Bruce Roe
• expression vectors for proteins   Rod Bonfiglioli
• lambda won't cut   chai_z at wehi.edu.au
• TYE Medium?   Michael Benedik
• Direct PCR of Lambda Libraries   READY at UTBC01.CM.UTEXAS.EDU
• HIV   BMA14432 at VAX1.UTULSA.EDU
  • HIV   Bruce Byrne
• AMMONIA CLEAVAGE FROM PROTEINS   BECKERM at SIMSC.EARN
• DNA methylation   Dr. Shoumo Bhattacharya B1 061
• Acidic polyacrylamide gels   Robert Horton
• mRNA from bovine PBMC   Robert Horton
• AMMONIA CLEAVAGE FROM PROTEINS   Robert Horton
• SDS PAGE, additives and elutions   Stuart E Ravnik
• N-ter labelling of proteins   Craig Justman
  • N-ter labelling of proteins   Keith Robison
• Cycle Sequencing Problems   batc at ac.dal.ca
• Lambda EMBL   Gabriel Trueba
• Lambda EMBL   Michael Benedik
• graft pcr, help needed********   Rod Bonfiglioli
• Chromosomes on disk(ette)?   Ari-Pekka Kvist
• correction to PCR message   Rod Bonfiglioli
• PCR1 and pSI 4001   Daryl Webb
• Lysis of bacteria   Bj|rn Forbord
• gene amplification   Dr Huaizheng Peng
• Help: Transfection into mammalian cells   Sean Davidson
• long-ranger gels?   Joel A. Kreps
• Epstein Barr   John Alsobrook
• PCR enhancers   John Alsobrook
• Epstein Barr   Bill Melchior, NCTR/FDA
• graft pcr, help needed********   Robert Horton
• commercial cDNA library   MBWEN at CCVAX.AS.EDU.TW
• Chromosomes on disk(ette)?   Heikki Lehvaslaiho
• Chromosomes on disk(ette)?   Heikki Lehvaslaiho
• Xho I: A Bad Actor?   bhjelle at carina.unm.edu
• Epstein Barr   Ashok Aiyar
• Xho I: A Bad Actor?   braam at DINGO.RICE.EDU
• direct phage sequencing   CALCIUM at FRCICT81.EARN
• graft pcr, help needed********   Basavaraju Shankarappa
• Sequence gel problems   afc at gnv.ifas.ufl.edu
• BclI   Mark D. Van Cleve
• p56lck   FGARBRECHT at mis.mcw.edu
• UV Shadowing   ggloor at kean.ucs.mun.ca
• Sequence gel problems   lev at DFCIAIDS.DFCI.HARVARD.EDU
• Re Bcl1   READY at UTBC01.CM.UTEXAS.EDU
• mRNA from fish liver   Johan deBoer
• BclI   Toby Bradshaw
• Xho I: A Bad Actor?   ww40
• expression vectors for proteins   Andrea Skantar
• UV Shadowing   DAVID F. BISHOP
• Re Bcl1   PHILLIP_SANMIGUEL
• BclI   Darren Natale
• Chromosomes on disk(ette)?   David A. Adler
• BclI   Darren Natale
• Re Bcl1   Michael Benedik
• Xho ligation problems   mangalam at SALK-SC2.SDSC.EDU
• Designing oligos for cDNA cloning   Yves Bertheau
• Acidic polyacrylamide gels   Yves Bertheau
• IEF Western blotting ok?   Yannick at CX13
• Macintosh Sequence Aligner/Editor?   Robert Rumpf
• PCR of ATCC samples   Christian Zwieb
• RT-PCR w/ cell lysates?   Darin O'Brien
• cm amplification   michael coyne
• Help secretion vectors   botdjl at lure.latrobe.edu.au
• Macintosh Sequence Aligner/Editor?   Bruce Roe
• colony hybridization   suter at VAX.MPIZ-KOELN.MPG.dbp.de
• dam- e.coli   Basavaraju Shankarappa
• cheap growth media other than Difco   Morris F. Manolson
• BclI   Mark D. Van Cleve
• BclI   Dennis J. Templeton
• cm amplification   Dennis J. Templeton
• BclI   Dennis J. Templeton
• looking for Mr. Bert Gold, Bob Distel, Ken Kleen   URA1335 at FRCITI51.BITNET
• Re Bcl1, and dam- strains   Pamela Norton
• dam- e.coli   Ashok Aiyar
• Re Bcl1, and dam- strains   Peter M. Muriana
• beta-lactamase assay, how and what substrate to use?   Tao Tao
• FACS of yeast   Stephen Kelley
• FACS of yeast   USA::RGB12955
• Getting serious about Lambda DNA.   the End
• cheap growth media other than Difco   Sylvain Chamberland
• cheap growth media other than Difco   Michael Benedik
• TAQ as reverse transcritase   RKOGEL at UCRVM2.BITNET
• chromosome 21   donald seto
• direct phage sequencing   Stuart Brown
• Direct PCR of Lambda Libraries   Stuart Brown
• Probing native DNA on filters(??)   Basavaraju Shankarappa
  • Probing native DNA on filters(??)   Cheung C. Yue
• chromosome 21   Basavaraju Shankarappa
• FACS of yeast   Darren Natale
• Cellophane   jhu at RESUNIX.RI.SICKKIDS.ON.CA
• FACS of yeast   Stephen Kelley
• Probing native DNA on filters(??)   GIDEON S. GORDON
• dam(n)- e.coli   Klaus v. Knoblauch
• GeneWorks   Robert Rumpf
• (none)   Prince of Wales Endocrinology Unit
• IEF Western blotting ok?   Yves Bertheau
• Cryofreezers (-20C) - any problems with yours?   Peter M. Muriana
• Cycle sequencing questions   batc at ac.dal.ca
• T cell epitopes   FRANK,BART
• MICROPIPETE CALIBRATION ?   WAGNER FONTES
• Table Top Centrifuge   DENNIS P. SMITH
• MICROPIPETE CALIBRATION ?   ww40
• MICROPIPETE CALIBRATION ?   the End
• Cryofreezers (-20C) - any problems with yours?   Klaus.Matthaei at anu.edu.au
• M13 Cloning   chai_z at wehi.edu.au
• Cryofreezers (-20C)   Bill Melchior, NCTR/FDA
• MICROPIPETE CALIBRATION ?   Al Binnie
• alignment, etc. software   Rick Wilson
• Northern with Digoxigenin   MIKE::PECHAN
• Probing native DNA on filters(??)   Paul N Hengen
• T cell epitopes   Dan Jacobson x-8453
• MICROPIPETE CALIBRATION ?   Tod Critchlow
• MICROPIPETE CALIBRATION ?   Bill Melchior, NCTR/FDA
• Acidic polyacrylamide gels   Robert Horton
• Cellophane   frist at ccu.umanitoba.ca
• MICROPIPETE CALIBRATION ?   lev at DFCIAIDS.DFCI.HARVARD.EDU
• MICROPIPETE CALIBRATION ?   Michael Benedik
• single strand specific mutagens ( >bisulfite)   Jim Graham
• Sequence gel problems   wetsel_r at wums.wustl.edu
• MICROPIPETE CALIBRATION ?   wetsel_r at wums.wustl.edu
• MICROPIPETE CALIBRATION ?   Mark Guiltinan
• Enzyme Reaction Rate   Jun Honda
• help secretion vectors   LOWDEN,Darren
• MICROPIPETE CALIBRATION ?   Anita Gould
• single strand specific mutagens ( >bisulfite)   Basavaraju Shankarappa
• MICROPIPETE CALIBRATION   DAVID F. BISHOP
• Clonetech's Bluemid   mball at ac.dal.ca
• PVDF as a Southern Support   fzhedric at hamlet.ucdavis.edu
• Ligation of Bcl1 & BamH1   navot1 at agri.huji.ac.il
• numbers of mRNA transcripts   Jiang Shunlin
• Dry Gel Hybridizations   bishop at MAP.MARC.USDA.GOV
• Enzyme Reaction Rate   Dr. R.J. Beynon
• Long Ranger (tm) gels   Number Ten Ox
• Long Ranger (tm) gels   ww40
• alignment, etc. software   Bruce Roe
• BclI and BamHI ligation   USA::AWS4270
• substitute PCR Gems   Bill Melchior, NCTR/FDA
• Leukocyte Marker absent in platelets   George W Chacko
  • Leukocyte Marker absent in platelets   bhjelle at carina.unm.edu
    • Leukocyte Marker absent in platelets   George W Chacko
• Xho I: A Bad Actor?   Ola Myklebost
  • Xho I: A Bad Actor?   A.K. Sweatman
• substitute PCR Gems   Nigel Walker
• MICROPIPETTE CLEANING   afc at gnv.ifas.ufl.edu
• UV Shadowing   bugg at mbcf.stjude.org
• transcription magic   Christian Zwieb
• MICROPIPETTE CLEANING   Klaus.Matthaei at anu.edu.au
• chloramphenicol amplification   mic225p at vaxc.cc.monash.edu.au
• non palindromic recognition sequences   Bill Melchior, NCTR/FDA
• Dratagene Affinity Screening Kit   Andy Phillips
• non palindromic recognition sequences   CLBGRM at PATERSON.CHRISTIE-HOSPITAL.MANCHESTER.AC.UK
• UV shadowing   Andy Phillips
• UV Shadowing   Dennis J. Templeton
  • UV Shadowing   Paul N Hengen
• Blot Name Conventions (was Probing native DNA on filters)   mangalam at SALK-SC2.SDSC.EDU
• Probing native DNA on filters(??)   Ed Rybicki
• Tritiated Chloramphenicol Source   Brian Brunk
• MICROPIPETE CALIBRATION ?   Paul N Hengen
• bionet-news   EXMED1::KARABINOS
• PCR enhancers   Dan Diaz
• Help is needed   batc at ac.dal.ca
• info needed on mutagenesis kit   Francis Ouellette
• PCR enhancers   Robert Horton
  • PCR enhancers   wetsel_r at wums.wustl.edu
• Cracked PAGE gels   BI1RGW at ibm.shef.ac.uk
• MICROPIPETTE CALIBRATION   Wagner Fontes
  • MICROPIPETTE CALIBRATION (FAQ contributions)   Paul N Hengen
• Promotor Database   Johan deBoer
• hot starts   scolnipa at esvax.dnet.dupont.com
• cracked PAGE gels   FGARBRECHT at mis.mcw.edu
• HOT-START   Basavaraju Shankarappa
• hot starts   kim at m44.unm.edu
• Xho I: A Bad Actor?   Thon de Boer
• Blot name conventions (was Probing native DNA on filters(??)   Cheung C. Yue
• SDS PAGE gels that never crack. Trust me.   Dennis J. Templeton
• Messages are received. Thanks a lot   batc at ac.dal.ca
• Looking for a good oligo design program. Help!   Marlon Pragnell
• Hybridization Incubators   Dave Knecht
• cm amplification?   michael coyne
  • cm amplification? (FAQ!!)   Paul N Hengen
• Immunoprecipitation (again!)   Andrew William Berry
• pcr gems, why use them ?   suter at VAX.MPIZ-KOELN.MPG.dbp.de
• BclI and BamHI ligation   Jarmo Niemi TUY
  • BclI and BamHI ligation   Dennis J. Templeton
• Looking for a good oligo design program. Help!   Basavaraju Shankarappa
• Looking for a good oligo   Dr. Shoumo Bhattacharya B1 061
  • Looking for a good oligo   rpgrant at vax.path.ox.ac.uk
• Televised Symposium: Luciferase as a Real-Time Reporter of Gene Expression   Thomas M. Breeden
• PCR Primer Design programs (longish)   Dan Jacobson x-8453
• Primer compilation   Richard J. Hughes
• Stratagene Quick-Hyb   kim at m44.unm.edu
• 33p   Steven J. Knapp
• Umea Drosophila stocks   Peter Rice
• Primer compilation   mangalam at SALK-SC2.SDSC.EDU
• Stratagene Quick-Hyb   Ed Rybicki
• Sequence of Bluescript   Martti Tolvanen
• cm amplification?   Tao Tao
• Photographing Coomassie-stained Gels   Peter M. Muriana
• Photographing Coomassie-stained Gels   Bill Melchior, NCTR/FDA
• Formaldehyde and DNA   Tod Critchlow
• microsatellites   van der Zeijst
• Cracked PAGE gels   kaya at mscf.med.upenn.edu
• Cracked PAGE gels   kaya at mscf.med.upenn.edu
• Photographing Coomasie-stained Gels   kaya at mscf.med.upenn.edu
• Sequence of Bluescript   Klaus.Matthaei at anu.edu.au
• LUCIFERASE EXPRESSION   Prince of Wales Endocrinology Unit
  • LUCIFERASE EXPRESSION   Sean Davidson
• Photographing Coomassie-stained Gels   Ed Rybicki
• Sequencing PCR Products - Best way?   Dr. Peter T. Boag
• hybridization problems   vewart at kean.ucs.mun.ca
• Summary: -20C Cryofreezer vs. Home freezers?   Peter M. Muriana
• Genebank on CDROM?   Robert Rumpf
  • Genebank on CDROM?   Dennis J. Templeton
• Size of exons and introns?   Dr. N.A. Affara
• cm amplification oof puc related plasmids   suter at VAX.MPIZ-KOELN.MPG.dbp.de
• p33   Steven J. Knapp
• re N's with DIG   Euan R. Taylor
• Genomic DNA purification system   inoue at ACS.UCALGARY.CA
• TA Cloning kits   Elli Wurtzel
• Genebank on CDROM?   Klaus.Matthaei at anu.edu.au
• hybridization problems   Klaus.Matthaei at anu.edu.au

Last message date: Wed Sep 30 18:30:22 EST 1992
Archived on: Sun Mar 6 19:45:36 EST 2005

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