Ecoli protein extracts

Jim Gale jim at AUDREY.LMF.ORG
Thu Apr 1 18:19:33 EST 1993


Greetigs Net Folk

I need to make crude protein extracts from Ecoli to assay for 
protein activity of a gene cloned into Bluescript SK- (isolated from
a cDNA expression library). What are your favorite methods for
making 'functional' protein extracts from Ecoli (ie no denaturents
used in prep that might kill protein activity). Also, this
plasmid is inducible with ITPG.

Thanks in advance

Jim
Lovelace Medical Foundation
Jim at audrey.lmf.org




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