lambda ZAPII, subtraction libraries?

Daniel Meier dmeier at
Fri Apr 2 16:37:58 EST 1993

Hello All,

In response to my inquiry about making lambda cDNA libraries, most people
recommended using lambda ZAP II as the vector.  Has anyone used ZAP II
vector/cDNA libraries in constructing subtraction libraries?  If so, 
can you send me a copy of your protocol?  If there are reasons for not
using ZAP II in subtraction libraries, please comment on that.
  Thanks much,

  Dan Meier
  dmeier at

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