"FAQ" -electroporation cuvettes, cleaning of.
vmiao at oregon.uoregon.edu
Mon Apr 5 02:16:25 EST 1993
In article <1993Mar31.093313.1 at molbiol.ox.ac.uk>, rpgrant at molbiol.ox.ac.uk
> I can't find this in the archive anywhere, so apologies for being thick.
> A while ago there was a discussion about cleaning electroporation cuvettes:
> The upshot was that it could be done, but I don't remember how. I
> did not save the result of the discussion :( Could someone give me a clue?
I didn't save the discussion either, although there were good suggestions,
but I wonder if you'd be interested in the way I clean cuvettes after
been used one for electroporating ligation mixes: Rinse out the cuvette
vigorously several times (e.g. 4x) with water, then rinse it out a couple
of times with ethanol. (Make sure you put the cap on and invert to rinse
off stuff that might be on the inside of cap). I decant the ethanol after
2nd time, leaving a few drops inside to evaporate during storage in the
until next use. That's all. I number the cuvettes and their caps, and
what DNA I used in them - just in case of trouble, at least I know what
be the most likely culprit. So far I haven't had any problems, and I've
reused some cuvettes 10 times or more. I'm not sure I would recommend
this perfunctory way of cleaning things when transforming with covalently
closed plasmids, since a little contamination would go a long way, but so
with the things I have been doing, it's been OK. :-)
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