mini prep procedure
jgraham at bronze.ucs.indiana.edu
Fri Apr 9 21:34:08 EST 1993
Oh, nothing personal of course. I just find your statement the ultimate example
of a trend these days.
As far as minipreps go, there are dozens of methods that produce DNA of
equivalent purity published in Biotechniques and Nucleic Acids Research every
year. If you have worked a "miniprep kit" into some kind of automated
column chromatography for one-step prep and sequencing then you might
be on the fast track, however, I would still find out what resin was involved
and how it works, and when I learned that that information was "proprietary",
junk it in favor of an equivalent one.
For minipreps that are as clean as Oliver North, take a look at Biotechniques
9(6) p.676 1990 (Shing-yi Lee and S. Rasheed) for starters.
PS. My point is that "special" columns in miniprep kits are not especially
reliable nor capable of producing DNA cleaner than the multitude of
refinements that are available for standard "bread and butter" minipreps
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