Oligo purification via urea gel??

John Alsobrook ALSOBROOK at biomed.med.yale.edu
Fri Apr 9 09:14:07 EST 1993


In <1q2b8v$du9 at usenet.INS.CWRU.Edu> bl275 at cleveland.Freenet.Edu writes:
>... 
> Forget the gels dude.  Purify your trityl-on oligos by reversed-phase HPLC.
> Detritylate, separate trityl and olig by another HPLC run.  Its fast, its
>...
> Dizzy Dan        Department of Molecular Genetics and Curried Sauces
>      Albert Einstein College of Medicine, Da Bronx, New York


Dan, a gel-box and power supply are well under $1000, and most mol.bio labs are
lousy with them... an HPLC set-up has how many zeroes in the price tag? HPLC
*IS* an excellent oligo prepper, but not everyone's got one, dude.


* John Alsobrook    Child Study Center    Yale University School of Medicine *
* internet: alsobrook at biomed.med.yale.edu / bitnet: alsobrook at yalemed.bitnet *
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* "Giving money and power to the government is like giving whiskey and car   *
*  keys to teenage boys." -- P.J. O'Rourke                                   *



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