Standardising Northerns

Dr. S. Bhattacharya sbhattac at crc.ac.uk
Tue Apr 13 10:28:37 EST 1993


Hi all,

I read somewhere that a 18S rRNA probe is a better option than the usual
actin for correcting loading variations on northern blots.  Does anyone have 
any ideas on this,
and has anyone done it using a oligonucleotide probe to 18s RNA (if so could 
they let me know the oligo sequence), as I do not have a cDNA probe.  

Many thanks

Shoumo Bhattacharya
MRC Molecular Medicine Group, 
RPMS, London 



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