Roller bottle hyb. ref.
bsh at MED.PITT.EDU
Thu Apr 15 07:50:25 EST 1993
I do not have the reference for roller bottles either. But I
thought it might be of some value for people using roller bottles\
to look at an other easy and cheap alternative.
You can buy a tube rotator, like the one you find in doctors office
or in Fisher catalog for about $150 This device rotateS tubes
in a full circle. All that needs to be done is punch some holes on
the surface of the disk and attach clamps that is suitable for say a
50 ml conical tube or bottles. At the least it is even possible to
tape the tubes or bottles. The next trick is in positioning the
rotator using proper supports in such a way that the tubes are held
at 90 degree angle when it is rotating. Now the whole assembly can be
placed inside a incubator maintained at the temperature you want. If you\
want a dedicated incubator, you can buy one cheap device for few hundred
dollars at the most. I think this is a considerable savings compared to
the few thousands you may have to shell out for the roller bottle
I will be interested to know if someone has tried similar approach.
bsh at med.pitt.edu
> Dear Netlanders;
> I am having difficulty finding a reference for the roller bottle
> technique for Southern/Northern hybridization. I know a lot of companies
> sell roller bottle hybridization machines, but I haven't seen a reference
> in their ads. Who invented this technique, and how do I reference 'em?
> Bob Horton /\ "Crash programs fail because of the theory that
> U. of Minnesota, CBS || with nine women pregnant you get a baby a month"
> 1479 Gortner Ave. /||\ -Werner von Braun. Disclaimer:"Bob who?"
> St. Paul, MN 55108 ^^ horton at molbio.cbs.umn.edu/(612) 624-3790
More information about the Methods