Detection limits of 32P ?

Daniel Mytelka mytelka at zenith.berkeley.edu
Fri Apr 16 17:15:21 EST 1993


In article <1993Apr16.100955.2335 at gnv.ifas.ufl.edu> afc at gnv.ifas.ufl.edu writes:
>In article <bheymann-150493171011 at biomac1p.bio.purdue.edu>, bheymann at bilbo.bio.purdue.edu (Bernard Heymann) writes:
>> In article <11857.jdboer at sol.uvic.ca>, jdboer at SOL.UVIC.CA ("Johan de Boer")
>> wrote:
>>> We have some dispute about how much 32P end-labelled DNA fragment can be
>>> detected by autoradiography. I am sure there is someone who has a pretty
>>> good idea. Any takers?
>>> 
>>> Johan de Boer
>> 
>The area that the signal occurs in is important. 1 CPM in a 1 micron spot
>would be easy to detect in an in situ.


My rough estimate is that this would work out to be about 40,000
molecules of DNA, assuming that it was 100% labelled.


Dan Mytelka
Dept of Molecular and Cell Biology, Berkeley
mytelka at mendel.berkeley.edu



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