Use of cycloheximide
DAC050 at MUVMS3.MU.WVNET.EDU
Sun Apr 18 08:47:00 EST 1993
I use cycloheximide at the concentration of 5 ug/ml. I have tried different
concentrations and this one works best for me on B16 melanoma cells.
Interestingly, the gene of our interest is up-regulated by cycloheximide at
10-20 fold higher than control. When I add 10 ug/ml of medium, there is
decrease in expression. We have done protein synthesis analysis by labelled
amino acid. Protein synthesis is inhibited at 5 ug/ml of medium.
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