linear DNA Xformation

skspoidn at reading.ac.uk skspoidn at reading.ac.uk
Mon Apr 19 04:42:20 EST 1993


It depends on why you want to do it.

My understanding is that Eukaryotic Xformations are done 
with linear DNA to improve the eficiency of integration into 
the chromosome. Since most bacterial Xformations involve
 autonomously replicating plasmids, linearizing the 
DNA would not serve much purpose. And if you actually wanted
 chromosomal integration, I would guess there are better ways
 than Xformation with linear DNA and then hoping :-)


Mike Poidinger
Dept of Microbiology
University of Reading
UK

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