Sequencing with Promega DNA

Andy baxevani at ncbi.nlm.nih.gov
Mon Apr 19 17:22:25 EST 1993


Has anyone had problems sequencing DNA purified using Promega's magic
column
miniprep kits? My results have been quite variable since I've started using
it,
with some samples sequencing beautifully and others not showing up on the
autorad at all -- I haven't experienced this kind of variability in the
past.
Of particular interest would be if any of you incorporate additional steps
prior to starting the sequencing reactions. I'm using USB's Sequenase to
run
the reactions.

Thanks in advance.



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