Sequencing with Promega DNA

Andy baxevani at
Mon Apr 19 17:22:25 EST 1993

Has anyone had problems sequencing DNA purified using Promega's magic
miniprep kits? My results have been quite variable since I've started using
with some samples sequencing beautifully and others not showing up on the
autorad at all -- I haven't experienced this kind of variability in the
Of particular interest would be if any of you incorporate additional steps
prior to starting the sequencing reactions. I'm using USB's Sequenase to
the reactions.

Thanks in advance.

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