transfected receptors ligand binding help

IBELGAUFTS at vms.biochem.mpg.de IBELGAUFTS at vms.biochem.mpg.de
Tue Apr 20 09:08:57 EST 1993


We have a stable cell line transfected with a receptor gene. The ligand binds
to this receptor. However we cannot get ligand binding in transient transfection assays. receptor expression in transient assays was confirmed by facs analysis
of an intracellular epitope tag coupled to the receptor. can it be that this molecule gets stuck in the golgi and is not expressed on the surface? 
Is anyone out there who has similar results or knows of similar ones
thanks for your help



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