PCR SPLICING

gough_k at wehi.edu.au gough_k at wehi.edu.au
Mon Apr 19 18:41:11 EST 1993


I am trying to splice PCR fragments together using the PCR.
I have two 1kbp fragments and want to join them togther by PCR.
The overlap at the ends is between 18 and 30 bp but I am getting 
variable results. Sometimes successful other times not so.
I have tried various temperatures, enzymes, DNA concentration
but with little help.
SOOOOOO HELP. Does anyone have any success?
Keith Gough, Melbourne, Australia.
Gough_K at WEHI.EDU.AU



More information about the Methods mailing list