Using RNase T2 for RPA

BOB RUTLEDGE BRUTLEDGE at AM.PNFI.FORESTRY.CA
Thu Apr 22 13:27:44 EST 1993


We have been unsucessfully attempting to use RNase T2 for 
RNase protection assay, according to a new protocol recently 
published in BioTechniques (BioTech 13, 847).  We suspect the 
procedure is failing because the T2 (BRL) is loosing its activity very 
early during the prescribed 60' digestion at 30 oC.

Has anyone tried this new protocol and/or can confirm our 
obseveration? 

Bob Rutledge
brutledge at pnfi.forestry.ca




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