PCR ss

Petur Henry Petersen php at rhi.hi.is
Thu Apr 22 16:11:19 EST 1993


	I am in PCR hell (again)... I am doing PCR on Cyt b in Cod.
I isolate the DNA with (prot. K, DTT, Phenol,iso-amyl:chl) and do
an ds PCR in it which usually is ok. However the asymmetric ss PCR
just suddenly did not work! I "cleaned" the ds DNA with Prot K and
extracted it again. That does sometime work and sometimes not...
Any suggestions or hins? If you have protocols for PCR and sequencing
please email me.

php at lif.hi.is
p.henry petersen
dept biology
U.iceland



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