Petur Henry Petersen
php at rhi.hi.is
Thu Apr 22 16:11:19 EST 1993
I am in PCR hell (again)... I am doing PCR on Cyt b in Cod.
I isolate the DNA with (prot. K, DTT, Phenol,iso-amyl:chl) and do
an ds PCR in it which usually is ok. However the asymmetric ss PCR
just suddenly did not work! I "cleaned" the ds DNA with Prot K and
extracted it again. That does sometime work and sometimes not...
Any suggestions or hins? If you have protocols for PCR and sequencing
please email me.
php at lif.hi.is
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