Help on quantitating a protein

Frederick Garbrecht fgarbrec at POST.ITS.MCW.EDU
Tue Apr 27 19:48:12 EST 1993


How about screening in an ELISA.  Maybe your antibody would be less
crossreactive against undenatured determinants.

F Garbrecht
Med Coll Wisconsin
fgarbrec at post.its.mcw.edu


On 27 Apr 1993, James Loats wrote:

> Date: 27 Apr 93 21:37:02 GMT
> From: James Loats <loats1 at husc8.harvard.edu>
> To: methods-and-reagents at net.bio.net
> Subject: Help on quantitating a protein
> 
> Hello all.  I am looking for a way to measure the expression levels of a
> protein in E. coli.  The problem is that antibodies to this protein (even
> ones made to synthetic peptides) are invariably cross-reactive with a great
> many other E. coli proteins, making for rather ugly Western blots.  What
> are some ideas that anyone has and their pros & cons (epitope tagging, etc.)
> 
> If more details are needed I am happy to provide them.  Post or e-mail
> 
> Jim L.
> 
> -- 
> _________________________________________________________________
> |Jim Loats                       |Bacterial Motor Works --      |
> |loats1 at husc.harvard.edu         |The Ultimate Swimming Machine!|
> -----------------------------------------------------------------
> 





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