removal of mineral oil

Stephanie Edelmann BIOSEE at ukcc.uky.edu
Wed Apr 28 16:17:07 EST 1993


In article <1993Apr28.165020.26430 at gserv1.dl.ac.uk>
skspoidn at reading.ac.uk writes:
 
>
>
>
>
>
>Well, Here is something that might work, based on a procedure
> I used to follow in eppendorfs....
>
>Try adding ether to the wells (to extract the oil) then freezing
> the samples in a dry ice bath. The ether should remian liquid,
> and can be flicked off, leaving your frozen aqueous 'pellet' in
> the bottom of the tray. Then just put in a fume hood for a few
>minutes to evaporate off the remaining ether. Voila!
>
>Of course, the ether might eat thru your microtitre tray, or your
> frozen aqueous 'pellets' might be a bit prone to being flicked out,
> but it *might* be worth a try :). Like I said, it works in eppendorfs.
>
>
>Mike Poidinger
>Dept of Microbiology
>University of Reading
>
>--- Keeping kit manufacturers in business ---
 
Don't know if this works for the original poster, but we remove the
oil by adding some chloroform to the eppendorff tube.  That causes the
mineral oil to sink to the bottom, the aqueous layer froms a bubble
on the top.
 
Stephanie



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