PCR/dot blot - removing mineral oil
Paul N Hengen
pnh at fcsparc6.ncifcrf.gov
Wed Apr 28 11:49:53 EST 1993
In article <MAILQUEUE-101.930428094810.171 at micro.uct.ac.za>
Ed Rybicki (ed at micro.uct.ac.za) writes:
>> To: methods-and-reagents at net.bio.net
>> From: al16 at cornell.edu (Adam Law)
>> Subject: PCR/dot blot - removing mineral oil
>> Date: 27 Apr 93 19:21:11 GMT
>> I am having problems removing mineral oil from PCR products prior to
>> application to a dot blot apparatus.
>There was a post some time back relating how to get rid of
>mineral/paraffin oil by pipetting your sample onto a piece of Parafilm and
>rolling it around until the oil had all stuck and only the aqueous phase
>was left. Can't remember who posted, but very cunning.
I was pretty sure John Nash had posted this, so I did a gopher search
for "mineral oil"...and sure enough:
|From: num208jn at MBDS.NRC.CA (John Nash)
|Subject: Re: Cetus' Gems
|Message-ID: <1992Feb26.142008.3174 at nrcnet0.nrc.ca>
|Date: 26 Feb 92 14:20:08 GMT
|A sales rep told me a neat trick to avoid chloroform extraction to
|remove mineral oil from PCR and linear-amp sequencing.
|Put the sample on a square of Parafilm, and the oil spreads out while
|the sample remains as a droplet. You can then pick it up with a
|micropipette, and you only lose a fraction of sample. (I usually get
|49 ul back from 50 .. end result ==> Chloroform? What chloroform?
Cheers to you too John!
Paul N. Hengen
National Cancer Institute
Frederick Cancer Research and Development Center
Frederick, Maryland 21702-1201 USA
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