Methods April 1993 Archives by date

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Starting: Thu Apr 1 03:27:29 EST 1993
Ending: Fri Apr 30 20:13:09 EST 1993
Messages: 396

Testis cDNA library Nir Navot
staining western blots for oligosaccharide David Martin x3175
Protein Data Bank Peter Rice
(none) HRMBDKC at HKUMD1.HKU.HK
staining western blots for oligosaccharide marder at agri.huji.ac.il
Nonradioactive labeling of DNA Peter Thoren
precipitation of NTPs Christian Zwieb
fusion/histidine/metal binding Roger Wiegand
Nonradioactive labeling of DNA Andre Hamel
SUMMARY: Your Choice of Reverse Transcriptase?????? Victor V. Levenson
unsub David Bradbury
PCR and Agarase Andre Hamel
precipitation of NTPs Andre Hamel
precipitation of oligos Jouko Kalevi Pettersson
Clontech strikes again 8-( Dawn Erin Kelley
chick ciliary ganglion cDNA library nishir at ohsu.edu
RT-PCR blues; help please! neale at mbcf.stjude.org
Urchin oocyte cDNA library (query) Christopher Kashina Patil
Ecoli protein extracts Jim Gale
Ecoli protein extracts rpgrant at molbiol.ox.ac.uk
RT-PCR from tissue? Johan de Boer
help maltose binding protein LOWDEN,Darren
Clontech strikes again FORNACE at FCRFV2.NCIFCRF.GOV
Testis cDNA library Michael Holland
staining western blots for oligosaccharide Michael Holland
PCR and Agarase Leslie Johnston-Dow
RT-PCR blues; help please! Andre Hamel
Urchin oocyte cDNA library (query) Dan Jacobson
Consistent subcloning from agarose (finally !) the End
single letter bases skspoidn at reading.ac.uk
lambda ZAPII, subtraction libraries? Daniel Meier
single letter bases John Nash
How do we remember what is what?... DAVID F. BISHOP
splicing genes with pcr jurgenson at iscsvax.uni.edu
splicing genes with pcr Vivian Miao
"FAQ" -electroporation cuvettes, cleaning of. Vivian Miao
Ecoli protein extracts Hugo Eric
Transcription Factor Binding Site - Summary Anthony Shearing
33P scolnipa at esvax.dnet.dupont.com
splicing genes with pcr Andre Hamel
Clontech Strikes: Clontech Response CLONTECH at BIOTECHNET.COM
33P Andre Hamel
33P Dennis J. Templeton
Consistent subcloning from agarose (finally !) Paul N Hengen
33P Melissa A. Melan
PCR and Agarase Klaus.Matthaei at anu.edu.au
Transient vs stable transfection regulation? Michael Holloway
fusion/histidine... Karl Fischer
RT-PCR blues; help please! Loryn Sellner
YAC sub-cloning Mr. A.R.J. Curtis
Consistent subcloning from agarose (finally !) Hugo Eric
How do we remember what is what?... Mark Siegal
Biomek for screening clones in microtiter plates (Q) Jari Vehmaanper{
Lactobacillus casei dihydrofolate reductase gene Hugo Eric
Site-directed Mutag. Kit Marc J. Mass
33P John Alsobrook
luminometer advice braam at DINGO.RICE.EDU
FISH pkt at canctr.mc.duke.edu
Lactobacillus casei dihydrofolate reductase gene Dan Jacobson
2nd Call: ICGEB Course on Oligonucleotides. FVEGA at CINVESMX.BITNET
Immobilized DNaseI Laura Via
Fairfield Chemical Stephen at imb.lan.nrc.ca
reuse Qiagen tips Fiberman
Immobilized DNaseI Basavaraju Shankarappa
Oligo purification via urea gel?? Tao Tao
Expr of fusion proteins in E. coli jwinkelman at vmsa.oac.uci.edu
Transient vs stable transfection regulation? Brain Foley
Oligo purification via urea gel?? Ashok Aiyar
Biotech Company Klaus.Matthaei at anu.edu.au
How do we remember what is what?... Hugo Eric
Bionet faq in Win3.1 help format, suggestions solicited Hugo Eric
Problems with 35-S-UTP from Amersham Timo Hiltunen
help needed with cosmid prep!!! Mr. A.F. Hill
help needed with cosmid prep!!! Mr. A.F. Hill
Speedvac w/ Aspirator? John J Welch
Oligo purification via urea gel?? Ashok Aiyar
help needed with cosmid prep!!! Andre Hamel
pBin19 sequence anywhere? David J. Meyer
pBin19 sequence anywhere? David J. Meyer
gamm-S-ATP and PK? Bob Gross
Consistent subcloning from agarose (finally !) the End
pBin19 sequence anywhere? Andre Hamel
reuse Qiagen tips erman at binah.cc.brandeis.edu
reuse Qiagen tips the End
Bionet faq in Win3.1 help format, suggestions solicited Ed Rybicki
reuse Qiagen tips Ed Rybicki
histidine-tagged proteins millerbs at LP.MUSC.EDU
luminometer advice BOB AT COMB
Carolina Workshop on In vitro Mutagenesis Philip L. Carl
Carolina Workshop on Transcription Philip L. Carl
mini prep procedure Kevin Kraus
mini prep procedure Kevin Kraus
RT-PCR OF PLANT MIT MRNA? FDONG at MIAMIU.BITNET
mini prep procedure the End
reuse Qiagen tips the End
33P Dan Diaz
Oligo purification via urea gel?? Dan Diaz
reuse Qiagen tips Dan Diaz
mini prep procedure Andre Hamel
mini prep procedure Marianna Max
Oligo purification via urea gel?? Bruce Roe
mini prep procedure Bruce Roe
Oligo purification via urea gel?? John Alsobrook
mini prep procedure the End
reuse Qiagen tips John Alsobrook
mini prep procedure Paul N Hengen
Transient vs stable transfection regulation? agoodrid at vaxa.weeg.uiowa.edu
mini prep procedure Marianna Max
AT to GC mutations Brian Margolin
IUBIO WAIS search of this group is a gold-mine! Brain Foley
Ligations. Restriction of PCR products with sites in primers. Brain Foley
mini prep procedure Martin Kennedy
mini prep procedure the End
mini prep procedure the End
IUBIO WAIS search of this group is a gold-mine! David Kristofferson
luminometer advice guo at mcclb0.med.nyu.edu
mini prep procedure Marianna Max
mini prep procedure the End
Hygromycin Vector for transformation of plants Mr. Xiaoying Lin
IUBIO WAIS search of this group is a gold-mine! Harry Mangalam
mini prep procedure Bruce Roe
Cloning in animals q00023 at TIGGER.STCLOUD.MSUS.EDU
Hygromycin Vector for transformation of plants BMA14432 at VAX1.UTULSA.EDU
no subject (file transmission) Ruth Finkelstein
In-line HPLC radioactivity detectors Mark D. Van Cleve
microdiffusion quantification of cyanide jp9787 at PANAM1.PANAM.EDU
gamma-S-ATP and PK? afc at gnv.ifas.ufl.edu
job placement BO124LIN at TWNAS886.BITNET
M13 and XL1-Blue SWANG at UAMS.bitnet
ColE1 plasmid w/ lacI(Q) ? the End
Paternity testing methodology. roger at kean.ucs.mun.ca
Standardising Northerns Dr. S. Bhattacharya
recover DNA from gels using agarase Hinayana Bawagan
degenerate PCR Dr. A. Rosenthal
HELP Where can I get a microscope bulb? Darrick Carter
degenerate PCR Andre Hamel
HELP Where can I get a microscope bulb? Michele Fuortes
AT to GC mutations delisior at rnisd0.DNET.roche.com
Detection limits of 32P ? Johan de Boer
RNA quantification & Northerns SSD4 at psuvm.psu.edu
RNA quantification & Northerns Daniel Meier
Standardising Northerns APPLING at UTBC01.CM.UTEXAS.EDU
Roller bottle hyb. ref. Robert Horton
recover DNA from gels using agarase (DON'T!!!!!) njhand at vax1.tcd.ie
cobra venom RNAase? Bob Gross
Bio101 RNaid Kits Foord, Brett
AT to GC mutations delisior at rnisd0.DNET.roche.com
Promega silver sequence...is it worth? Richard J. Hughes
Methylene blue to stain agarose gels srckbjg at chv.lincoln.cri.nz
quantitating silver gels? Philip Joseph Moos
RNA quantification & Northerns Rosemary Hoffman
Promega silver sequence...is it worth? Dan Diaz
HELP - E.coli strains Wayne Woods
Roller bottle hyb. ref. Basavaraju Shankarappa
Colorimetric substrate for RNAse? Roger Wiegand
Anyone EVER successful with Demmler's PCR on Cytomegalovirus? REE700A at MAINE.MAINE.EDU
YAC sub-cloning Ola Myklebost
Gel slice DNA recovery. JON BOXALL.
AT to GC mutations the End
Gel slice DNA recovery. the End
batrachotoxin - what where how ? R.G. Walters
Standardising Northerns Ola Myklebost
Tomato cell culture line SERRES at UCRAC1.UCR.EDU
histone deacetylase Jonathan.Walton
gamma-S-ATP and PK? Bob Gross
AT to GC mutations the End
HELP - E.coli strains Michael Benedik
HELP - E.coli strains GIETZ at bldghsc.lan1.umanitoba.ca
Detection limits of 32P ? Bernard Heymann
Roller bottle hyb. ref. Klaus.Matthaei at anu.edu.au
New prototype newsgroup for YACs Dave Kristofferson
HELP - E.coli strains Tao Tao
Colorimetric substrate for RNAse? ctwittwer at msscc.med.utah.edu
Colorimetric substrate for RNase EM609MBCPHL at NCCCOT7.AGR.CA
Cobra Venom RNAse EDWIN TEN DAM
Radiolabelled adenine analogues? Johan de Boer
Subcloning of YACs Ola Myklebost
Clones genes that don't express Doon
Filtering Buffers... Daniel Meier
Detection limits of 32P ? afc at gnv.ifas.ufl.edu
Help with lambda zap excision coutavas at mcclb0.med.nyu.edu
variation in transfection efficiency Fiona Fairhurst
RNA from skin tissue cdds01 at vaxa.strath.ac.uk
pMamNeo from Clontech vaughan at sci.wfeb.edu
Help with zap excision, part II coutavas at mcclb0.med.nyu.edu
Detection limits of 32P ? Daniel Mytelka
Promega silver sequence...is it worth? Mu Jianlong
variation in transfection efficiency Andre Hamel
Filtering Buffers... Andre Hamel
Help with lambda zap excision Andre Hamel
RNA from skin tissue Andre Hamel
splicing genes with pcr Robert Wildin
AMD Beth Stewart
M13 and XL1-Blue Bruce Roe
Standardising northerns Dr. S. Bhattacharya
RNA from skin tissue Ola Myklebost
RNA quantification & Northerns Euan R. Taylor
Use of cycloheximide Racine Jean-Francois
Back to back PCR help erman at binah.cc.brandeis.edu
Help with lambda zap excision Martin Kennedy
pgm clone needed michael coyne
Use of cycloheximide Dinakar Desai
Vent polymerase ? the End
Vent polymerase ? Vivian Miao
Back to back PCR help Vivian Miao
Filtering Buffers... Euan R. Taylor
variation in transfection efficiency guo at mcclb0.med.nyu.edu
linear DNA transformation bacteria help IBELGAUFTS at vms.biochem.mpg.de
lysis of YAC containing yeasts help IBELGAUFTS at vms.biochem.mpg.de
linear DNA Xformation skspoidn at reading.ac.uk
Vent polymerase ? Andre Hamel
linear DNA transformation bacteria help Andre Hamel
Terminology skspoidn at reading.ac.uk
cloned genes: no expression Doon
Terminology Andre Hamel
linear DNA Xformation Patricia Foster
Direct Sequencing of Genomic DNA Jerry Salem
whole mount in situ--background problem Hung Fang
PFGE/FIGE with denatured DNA gkantor at desire.wright.edu
linear DNA Xformation Michael Benedik
Sequencing with Promega DNA Andy
test gough_k at wehi.edu.au
linear DNA Xformation Paul N Hengen
PCR SPLICING gough_k at wehi.edu.au
Terminology Russell A. Maurer
maxi-cell procedures Donald Chen - Microbiology
Maniatis Karyn Davis
Site directed mutations Jeffrey Alan Silverman
Labeled PCR products Ken Krauter
transfected receptors ligand binding help IBELGAUFTS at vms.biochem.mpg.de
whole mount in situ--background problem Andre Hamel
Sequencing with Promega DNA Andre Hamel
PCR SPLICING Andre Hamel
Labeled PCR products Andre Hamel
Electroporation of Pseudomonas syringae Reon Brand
Ethidium Bromide mutagenesis Christopher T. Cole
Terminology Michael van Waes
Information on pHomer Keith Robison
Sequencing with Promega DNA USA::RGB12955
PCR primers for genetic studies Bob Sinclair
Need help with IEF gab at gnv.ifas.ufl.edu
PCR short primer products ? the End
Sequencing with Promega DNA Andre Hamel
In Situ Hybridizations of Seeds Andre Hamel
Help me compile a transfection FAQ: Brain Foley
In Situ Hybridizations of Seeds mmelan at pattie.wellesley.edu
Pads for Westerns . MJE100G at ODUVM.BITNET
commercial sequencing services Alberto I Roca
Terminology Ed Rybicki
Ethidium Bromide mutagenesis bickle at urz.unibas.ch
whole mount in situ--background problem Christophe Roos
HHV6 human herpesvirus 6 contacts wanted IBELGAUFTS at vms.biochem.mpg.de
amino acid sequence of yeast MAT alpha-2 ? lhanson
Ethidium Bromide mutagenesis Gordon G. Churchward
Need help with IEF Andre Hamel
Best E. Coli? Craig Morton
PCR short primer products ? Andre Hamel
fusion proteins LYNDON at BMS-UNIT.ICR.AC.UK
amino acid sequence of yeast MAT alpha-2 ? Francis Ouellette
splicing genes with pcr Bob DeLisio
WAS: silver sequence/ Promega Craig Justman
amino acid sequence of yeast MAT alpha-2 ? Dan Jacobson
Colorimetric substrate for RNAse? Michael S. Kay
In situ hybs in Human Platelets George W Chacko
DNA fluorometers EM609MBCPHL at NCCCOT7.AGR.CA
How does silver staining work? ctwittwer at msscc.med.utah.edu
etbr mutagenesis john hachey
Terminology Andre Hamel
Terminology skspoidn at reading.ac.uk
Rat cell line: adrenal gl./cortical cells Christophe Roos
Need help with IEF David J. Meyer
nuclear run on Kathleen Hopp
RSV expression vector needed Robert Horton
rat cells skspoidn at reading.ac.uk
<None> gkantor at desire.wright.edu
Quick PCR Patricia Foster
Consistent subcloning from agarose (finally !) Paul N Hengen
Using RNase T2 for RPA BOB RUTLEDGE
p-tyr blotting question Frederick Garbrecht
Quick PCR Bob DeLisio
PCR ss Petur Henry Petersen
Quick PCR Klaus.Matthaei at anu.edu.au
Quick PCR George W Chacko
Quick PCR Andre Hamel
Western Blotting of Membrane Proteins in the presence of SDS Shaun D. Black
pHomer kpf at bones.biochem.ualberta.ca
on Kc cells ZUNINO at MVX36.CSATA.IT
dye staining of agarose gels Mr. A.F. Hill
DNA content of microorganisms Peter Alefounder
Iso-Electric Focusing - HELP Joan Shields
Beta-Mercaptoethanol Storage Jeffrey Alan Silverman
heparin-sepharose chromatography for bFGF Joy Richman
Promega PolyATtract mRNA Isolation System Christopher T. Huhta
Quick PCR Paul N Hengen
Quick PCR Paul N Hengen
KLH for peptide conjugation Richard J. Hughes
Western Blotting of Membrane Proteins in the presence of SDS Marivonne
Quick PCR Mike Morris
commercial sequencing services Howard Cash
Quick PCR Bill Melchior
DNA fluorometers gkantor at desire.wright.edu
field isolation protocols for HMW DNA? steven heckt
cycle sequencing from mini prep DNA Joy Richman
cycle sequencing from mini prep DNA Andre Hamel
Western Blotting of Membrane Proteins in the presence of SDS James B. Hutchins
Quick PCR Martin Kennedy
Promega PolyATtract mRNA Isolation System Martin Kennedy
unexpressed genes Doon
Western Blotting of Membrane Proteins in the presence of SDS James B. Hutchins
Pipettor reviews John H McDonald
Conway Microdiffusion Method jp9787 at PANAM1.PANAM.EDU
pCR-Script (Stratagene) cloning kit experience? Zoltan Penzes
Help with lambda zap excision jasper at molbiol.ox.ac.uk
PolyA+ purification with oligodT cellulose srcksac at chv.lincoln.cri.nz
Program for protein binding sites Hirdeypal S. Bhathal
pHomer Keith Robison
lysis of YAC containing yeasts help Anne Forus
ionophoretic antibiotics DOSIO at ch.unito.it
sequence motifs specifying unsoluble proteins? IBELGAUFTS at vms.biochem.mpg.de
Quick PCR k_garson at icrf.icnet.uk
GenHunter SEPPO KAIJALAINEN MMETT
Southerns-- Ethidium Bromide Daniel Meier
Consistent subcloning from agarose (finally !) the End
GenHunter ww40
What is "takadiastase"? William Brown
Promega PolyATtract mRNA Isolation System Robert Horton
Western Blotting of Membrane Proteins in the presence of SDS millerbs at LP.MUSC.EDU
p-tyr blotting question Marivonne
Southerns-- Ethidium Bromide Klaus.Matthaei at anu.edu.au
DNA Strider Bob DeLisio
help! how to pack a sepharose column Cathy Quinones
Luciferase reporter Klaus.Matthaei at anu.edu.au
test Marilou Andres
CsTFA gradients Antonio R. Franco
Problems with sequencing Antonio R. Franco
DNA Strider Harry Mangalam
test James N Petitte
Large scale plasmid preps James N Petitte
LMP agarose from FMC Nelson Salazar
pogs (milk bottle caps) JOHN
p-tyr blotting question bhjelle at carina.unm.edu
PCR/dot blot - removing mineral oil Adam Law
Large scale plasmid preps Dinakar Desai
DNA Strider David Stillman
DNA Strider David Stillman
DNA Strider David Stillman
PCR/dot blot - removing mineral oil Darren Natale
InterSpex Novozyme 234 R.D. Johnson
Help on quantitating a protein James Loats
PCR/dot blot - removing mineral oil Cheung C. Yue
Large scale plasmid preps Andre Hamel
PCR/dot blot - removing mineral oil Andre Hamel
PCR/dot blot - removing mineral oil Andre Hamel
Help on quantitating a protein Frederick Garbrecht
PCR/dot blot - removing mineral oil Ed Rybicki
da cells what are they? IBELGAUFTS at vms.biochem.mpg.de
p-tyr blotting question Berthe Marie Willumsen
nuclei isolation from rice leaves BAO at JII.AFRC.AC.UK
LARGE SCALE PLASMID PREPS DUNCKLEY at RESA.AFRC.AC.UK
PCR/dot blot - removing mineral oil bhjelle at carina.unm.edu
p-tyr blotting question Euan R. Taylor
PCR/dot blot - removing mineral oil Paul N Hengen
removal of mineral oil skspoidn at reading.ac.uk
nuclei isolation from rice leaves BOB RUTLEDGE
References on epitope tagging James Loats
removal of mineral oil Stephanie Edelmann
help! how to pack a sepharose column James B. Hutchins
PCR/dot blot - removing mineral oil the End
Colorimetric Nuclease Detection Jennifer L Liedtke
Promega non-isotopic sequencing kit quinn at icbr.ufl.edu
What is "takadiastase"? Ragnar
Is quick PCR suitable for detecting chromosomal alterations? Tao Tao
test Dr. G. Koraimann
cycle sequencing from mini prep DNA Euan R. Taylor
Programs for image analysis of gels? Martin Ligr
PCR/dot blot - removing mineral oil Andre Hamel
PCR/dot blot - removing mineral oil Andre Hamel
need a connective tissue cell line BURROWS at HELIX.MGH.HARVARD.EDU
Make-your-own dNTPs? Robert Horton
Help on quantitating a protein pbonham at pbi.nrc.ca
PCR/dot blot - removing mineral oil Klaus.Matthaei at anu.edu.au
help! how to pack a sepharose column Corinne L Reimer
microtiter plate PCR? EM609MBCPHL at NCCCOT7.AGR.CA
microtiter plate PCR? EM609MBCPHL at NCCCOT7.AGR.CA
help! how to pack a sepharose column hUnix
quantitative westerns HRMBDKC at HKUMD1.HKU.HK
microtiter plate PCR Rick Wilson
whole mount in situs Bill
Don't you need to degas the sepharose? Basavaraju Shankarappa
DNA content of microorganisms Dan Diaz
PCR/dot blot - removing mineral oil Paul N Hengen
PCR/dot blot - removing mineral oil Patricia Foster
PLEASE READ - IMPORTANT NEW SERVICE(S) FROM BIOSCI!!!! Dave Kristofferson
Large scale plasmid preps nishir at ohsu.edu
PCR/dot blot - removing mineral oil Andre Hamel
help! how to pack a sepharose column David J. Meyer

Last message date: Fri Apr 30 20:13:09 EST 1993
Archived on: Sun Mar 6 19:48:42 EST 2005

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