I am doing SSCP at moment. I use routine method to denature DNA fragemnt (95% formamide,0.01%dye, equal volume dilute PCR reaction products,boil 5 min ,and then load on 6-10% gel). I detect dsDNA and ssDNA by Ag staining. I can not denature DNA prope
PS: I tried add some alkaline into loading mixture, it didnot work.