denature in SSCP

Dr Huaizheng Peng rmkdhhp at ucl.ac.uk
Mon Aug 2 05:48:41 EST 1993


Hi, group,

I am doing SSCP at moment. I use routine method to denature DNA fragemnt (95% formamide,0.01%dye, equal volume dilute PCR reaction products,boil 5 min ,and then load on 6-10% gel). I detect dsDNA and ssDNA by Ag staining. I can not denature DNA prope

PS: I tried add some alkaline into loading mixture, it didnot work.

Thank you.

PHZ



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