TA cloning vector

RICHARD P. PHIPPS PHIP at BPHVAX.BIOPHYSICS.ROCHESTER.EDU
Tue Aug 3 15:53:33 EST 1993


Hello, net, 

I'll be doing some cloning of PCR products in the near future and I was 
planning on using TA cloning as described by Invitrogen.  I was hoping, 
however, not to have to buy their kit.  Does anyone know of a vector 
that is easily obtained and can be used for this purpose (i.e., can be 
cut with an enzyme which leaves a single T overhang).  Thanks in advance,
I'll post a summary later.

Rick Willis 
University of Rochester Cancer Center



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