Boiling Method for Minipreps (CTAB variation)


Tue Aug 3 14:12:48 EST 1993


In article <23lvng$le at usenet.INS.CWRU.Edu> djt2 at po.CWRU.Edu (Dennis J.  
Templeton) writes:
> Transfer to a microfuge tube. No deliberate incubation is needed.  Boil 1.5
> minutes, spin 15,000 rpm 10 minutes, pluck out pellet with toothpick.  Fill
> tube with 0.5% CTAB in water (warm to make sure it is dissolved) from a  
squirt

Two questions:

1) What is CTAB?

2) Will the heat resistant nuclease problem associated with 
   HB101 be taken care of by a Phenol/Chloroform extraction
   before the EtOH ppt. step in the standard boiling mini prep
   protocol?

Thanks

Rick Lynch
Agouron Institute
rlynch at vaxkiller.agi.org



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