Boiling Method for Minipreps (CTAB variation)
Tue Aug 3 14:12:48 EST 1993
In article <23lvng$le at usenet.INS.CWRU.Edu> djt2 at po.CWRU.Edu (Dennis J.
> Transfer to a microfuge tube. No deliberate incubation is needed. Boil 1.5
> minutes, spin 15,000 rpm 10 minutes, pluck out pellet with toothpick. Fill
> tube with 0.5% CTAB in water (warm to make sure it is dissolved) from a
1) What is CTAB?
2) Will the heat resistant nuclease problem associated with
HB101 be taken care of by a Phenol/Chloroform extraction
before the EtOH ppt. step in the standard boiling mini prep
rlynch at vaxkiller.agi.org
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