ATCC Data now available by Gopher

Dan Jacobson danj at welchdev.welch.jhu.edu
Mon Aug 2 18:02:29 EST 1993


You can now access data from the American Type Culture Collection (ATCC)
via gopher.

Point your gopher client at merlot.welch.jhu.edu and select:

12. Search Databases at Hopkins (Vectors, Promoters, NRL-3D, EST, OMIM./

   -->  6.  Culture Collections (ATCC)/

    -->  1.  ATCC - The American Type Culture Collection/

Or point your gopher client at atcc.nih.gov and select:

 -->  1.  ATCC Catalogs (courtesy of Johns Hopkins University)/


Either way you'll see:

--> 1.  Information from and about the ATCC/
    2.  Bacteria and Bacteriophages/
    3.  Cell Lines and Hybridomas/
    4.  Protozoa and Algae/
    5.  Animal and Plant Viruses and Antisera/
    6.  Fungi and Yeasts/
    7.  Recombinant DNA Materials (Clones, Vectors, Libraries, Hosts, Oligo/

Here you will find a number databases which you can search in order to find 
strains, cell lines or recombinant materials of interest as well as 
information about the ATCC, ordering information, and an online order form.


To search for strains of bacteria select:

 -->  2.  Bacteria and Bacteriophages/

     -->  1.  Bacteria <?>

and enter a word or series of words that describe your interests.  You will
then see a list of articles that have at least one of the words you typed
in.

You can further modify your search by use of the words 'and' and 'not'.
For example:

coli and streptomycin

will select articles which contain both the word 'coli' and the word
'streptomycin'

A typical entry retrieved by such a search is:

----------------------------------------------------------------------

Escherichia coli
ATCC 25250

Description:

Preceptrol [Reg TM] culture. N.M. Schwartz 13-6. [E.A. Adelberg AB
1515] Derived from Escherichia coli K-12. F-; requires vitamin B1,
leucine, proline, adenine and tryptophan for growth; resistant to
phage T6, streptomycin and sodium azide; unable to ferment lactose
(lacY), arabinose, xylose and mannitol; does not carry phage lambda
and is resistant to lambda (N.M. Schwartz, personal communication).

Growth Conditions: Medium 3  37C

Shipped: Freeze-dried.

Price Code: P


------------------------------------------------------------------------

Now you see that the Growth Conditions are Medium 3  37C - so to look
up Medium 3 select:


 -->  3.  Media Formulations <?>

and search for 

"Medium 3 "

Note here that there are qoutes around the search term - this is
called a phrase search and is important to use when searching for
Medium numbers.  The Medium entries are recipes and as such have lots
of numbers in them - so if you are searching for a specific Medium
number you should always put the phrase in quotes.

However you may search the Media Formulations for ingredients in
addition to identification numbers.  For example if you were looking
for a Heart infusion broth with streptomycin you would search for:

heart and streptomycin

and you would locate the following Medium:

-----

ATCC Culture Medium 1095 HEART INFUSION BROTH with 0.1% GLUCOSE and
ANTIBIOTICS

Description:

Heart Infusion Broth (Difco 0038), 25.0 g
Glucose*, 1.0 g
Streptomycin sulfate*, 100.0 mg
Tetracycline HCl*, 25.0 mg
Distilled water, 1.0 L
*Filter-sterilize.

-----


You may also use wildcards (*) to search for partial words.  If you
wanted to search for the words - mutate, mutated, mutates, mutation, 
mutations ... you would simply use:

mutat*

The Bacteria section has been used as an example here - the instructions
apply to all the other data sets (Cell Lines and Hybridomas, Protozoa
and Algae, Viruses and Antisera, Fungi and Yeasts, and Recombinant DNA
Materials) as well.


Happy searching,

Dan Jacobson

danj at welchgate.welch.jhu.edu

Johns Hopkins University



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