plant RNA extraction method
RSCOTT at IRRI.CGNET.COM
Thu Aug 5 01:05:01 EST 1993
> From: IN%"<@CGNET.COM:browns at ccu.umanitoba.ca>" "browns" 5-AUG-1993 05:47
> We have been using a classic method using phenol and LiCl
> in a Tris/SDS grinding buffer, followed by chloroform
> extractions, 2M LiCl precipitation, and EtOH pptn.
> This gives fair to poor results with lots of sample-to-sample
> and day to day variability.
> I just tried out a guanidium thiocyanate method with very poor
> resuslts- basically my first isopropanol pellet (after 1st
> chloroform extraction) contained a huge amount of junk which
> refused to redissolve.
> I need a plant method that is fairly quick and amenable to
> scale up for handling lots of samples per day.
A few days ago Janet Braam <braam at dingo.rice.edu> suggested the following
quick protocol to a similar inquiry:
Verwoerd et al. (1989) Nucl. Acids Res. 17: 2362.
It might work for you. Good luck!
Intl Rice Res Inst
rscott at irri.cgnet.com
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