plant RNA extraction method

RPSCOTT RSCOTT at IRRI.CGNET.COM
Thu Aug 5 01:05:01 EST 1993


> From:	IN%"<@CGNET.COM:browns at ccu.umanitoba.ca>"  "browns"     5-AUG-1993 05:47
> 
> We have been using a classic method using phenol and LiCl    
> in a Tris/SDS  grinding buffer, followed by chloroform
> extractions, 2M LiCl precipitation, and EtOH pptn.
> This gives fair to poor results with lots of sample-to-sample
> and day to day variability.
> 
> I just tried out a guanidium thiocyanate method with very poor
> resuslts- basically my first isopropanol pellet (after 1st 
> chloroform extraction) contained a huge amount of junk which
> refused to redissolve.
> 
> I need a plant method that is fairly quick and amenable to
> scale up for handling lots of samples per day.
> 


A few days ago Janet Braam <braam at dingo.rice.edu> suggested the following
quick protocol to a similar inquiry:
	

	Verwoerd et al. (1989) Nucl. Acids Res. 17: 2362.

It might work for you. Good luck!

R. Scott
Intl Rice Res Inst
Philippines
rscott at irri.cgnet.com

  



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