Boiling Method for Minipreps

Jim Owens jow at helix.nih.gov
Wed Aug 4 17:19:47 EST 1993


In article <9308032222.AA20703 at calshp.cals.wisc.edu> ,
jchen at CALSHP.CALS.WISC.EDU writes:
>As a matter of fact, I prefer HB101 as a
>host because cells are easy to resuspend into the lysozyme buffer.

Somewhere in my collection of references there is a report about lysozyme
not being necessary, and even reducing plasmid yield, in some strains of
_E. coli_.  So I could not help wondering if anyone has tried a boiling
miniprep without adding lysozyme.

I personally use the alkaline lysis method modified somewhat, but without
lysozyme, for mini-, midi- and maxipreps of plasmids.

Good luck,

Jim Owens



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