In article <1993Aug3.182802.1 at molbiol.ox.ac.uk>, nickerson at molbiol.ox.ac.uk (Darren Nickerson) writes:
>> Would anyone care to comment on the apparatus/techniques they use to
> autoradiograph S35 radiolabelled gels which have been dried onto the plate of
> Bio-Rad's IPC sequencing setup? AT the moment we use a black blanket and
> black garbage bags, pressing the film firmly between the front plate (with gel
> affixed) and the back plate, or IPC buffer-tank. This takes the back plate
> out of action for a bit, so we are currently looking for a more elegant way of
> doing it. We have had no luck finding a casette which will hold the plate,
> and so have resorted to the above procedure. Can anyone suggest a supplier,
> or a better home remedy??? :)
>> Darren Nickerson
We just take our IPC's apart, don't fix the gel, lay a sheet of 3MM cut to same
size as gel, press it on gently and then peel the whole lot off, cover with
plastic wrap and dry in a gel dryer.
Cheers,
Martin
NNNN NN Martin A Kennedy (E-mail = mkennedy at chmeds.ac.nz) ZZZZZZZ
NN NN NN Cytogenetic and Molecular Oncology Unit ZZZ
NN NN NN Christchurch School of Medicine ZZZ
NN NNNN Christchurch, New Zealand ZZZZZZZ
Phone (64-3)364-0880 Fax (64-3)364-750
