Autorad. Cass. for Bio-Rad IPC????

Martin Kennedy mkennedy at chmeds.ac.nz
Wed Aug 4 16:54:09 EST 1993


In article <1993Aug3.182802.1 at molbiol.ox.ac.uk>, nickerson at molbiol.ox.ac.uk (Darren Nickerson) writes:
> 
> 	Would anyone care to comment on the apparatus/techniques they use to 
> autoradiograph S35 radiolabelled gels which have been dried onto the plate of 
> Bio-Rad's IPC sequencing setup?  AT the moment we use a black blanket and 
> black garbage bags, pressing the film firmly between the front plate (with gel 
> affixed) and the back plate, or IPC buffer-tank.  This takes the back plate 
> out of action for a bit, so we are currently looking for a more elegant way of 
> doing it.  We have had no luck finding a casette which will hold the plate, 
> and so have resorted to the above procedure.  Can anyone suggest a supplier, 
> or a better home remedy???  :)
> 
> Darren Nickerson

We just take our IPC's apart, don't fix the gel, lay a sheet of 3MM cut to same
size as gel, press it on gently and then peel the whole lot off, cover with
plastic wrap and dry in a gel dryer.


Cheers,

Martin

NNNN   NN  Martin A Kennedy (E-mail = mkennedy at chmeds.ac.nz)  ZZZZZZZ  
NN NN  NN       Cytogenetic and Molecular Oncology Unit          ZZZ
NN  NN NN           Christchurch School of Medicine            ZZZ
NN   NNNN              Christchurch, New Zealand              ZZZZZZZ
		Phone (64-3)364-0880  Fax (64-3)364-750



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