Brian Halligan (halligan at post.its.mcw.edu) wrote:
: I have heard that Northern Blots can be stained with methylene blue before
: hybridization to obviate the need for EtBr staining. Does anyone have a
: protocol or opinions on this?
I found this method but have not tried it yet. I ran across it but neglected
to write the reference.
Staining of rRNA bound to membranes
Immerse membrane briefly in 0.03% methylene blue, 0.3 M NaOAc pH 5.2
Destain in water
Hybridize as usual