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Methylene Blue staining

fzestabr at bullwinkle.ucdavis.edu fzestabr at bullwinkle.ucdavis.edu
Wed Aug 4 13:52:11 EST 1993

Brian Halligan (halligan at post.its.mcw.edu) wrote:

: I have heard that Northern Blots can be stained with methylene blue before
: hybridization to obviate the need for EtBr staining.  Does anyone have a
: protocol or opinions on this?
Dear Brian

I found this method but have not tried it yet. I ran across it but neglected
to write the reference.

Staining of rRNA bound to membranes
Immerse membrane briefly in 0.03% methylene blue, 0.3 M NaOAc pH 5.2
Destain in water
Hybridize as usual

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