subject: EcoRI site in lacZ gene
The lacZ gene from E. coli contains an EcoRI site near the very 3' end of the
gene. In a number of vectors including YEp353, pmC1843, and pSKS104, the 3'
end of the gene no longer contains this EcoRI site. I traced the mutation back
to a reference Casadaban et. al. J. bact 143:971 (1980) where they transduced a
phage into a strain containing the lacZ gene on a plasmid to create an EcoRI
mutation that did not affect B-galactosidase function. They created the vector
pMC1403. The above vectors are derivatives of pMC1403. This reference did
not describe the nature of the EcoRI mutation. Does anybody know the
sequence of this mutated site or a reference which describes the sequence of
this mutated site?
e-mail address: Stillmanlab at Bioscience.Utah.edu